中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2010年
1期
21-23
,共3页
邓敏%李明慧%刘顺爱%刘凤%桑燕%宋淑静%张书凤%关小平%谢尧%邬小萍%成军%徐道振
鄧敏%李明慧%劉順愛%劉鳳%桑燕%宋淑靜%張書鳳%關小平%謝堯%鄔小萍%成軍%徐道振
산민%리명혜%류순애%류봉%상연%송숙정%장서봉%관소평%사요%오소평%성군%서도진
肝炎,乙型,慢性%T淋巴细胞%受体,白细胞介素7%病毒载量
肝炎,乙型,慢性%T淋巴細胞%受體,白細胞介素7%病毒載量
간염,을형,만성%T림파세포%수체,백세포개소7%병독재량
Hepatits B,chronic%T-lymphocytes%Receptors,interleukin-7%Viral load
目的 探讨慢性HBV感染者外周血中CD4~+CD25~+Foxp3~+调节性T细胞(RegulatoryT,Treg)的水平,并研究其两种细胞标志--CD127分子的低表达与Foxp3转录因子表达之间的关系.方法 采集34例免疫耐受期、26例免疫清除期和31例非活动或低(非)复制期慢性HBV感染者的外周全血,用流式细胞术分析CD4~+CD25~+Foxp3~+T细胞和CD4~+CD25~+CD127~(low)T细胞的频率.结果 在慢性HBV感染者外周血中,免疫耐受组CD4~+CD25~+Foxp3~+T细胞和CD4~+CD25~+CD127~(low)T细胞的频率均比非活动或低(非)复制组高(Z=-2.693,P=0.007和t=3.251,P=0.002);且病毒阳性组(免疫耐受组+免疫清除组)比非活动或低(非)复制组高(t=2.266,P=0.026和t=3.208,P=0.002);ALT异常组(免疫清除组)与ALT正常组(免疫耐受组+非活动或低(非)复制组)之间的比较差异无统计学意义(P>0.05).91例病例外周血中CD4~+CD25~+Foxp3~+T细胞和CD4~+CD25~+CD127~(low)T细胞之间的表达有一致性,但两者频率的差异有统计学意义(Z=-4.718,P<0.001).结论 调节性T细胞在慢性HBV感染者病毒高复制组中明显升高.CD4~+CD25~+T细胞中CD127分子的低表达与Foxp3转录因子的表达有一致性,但前者代表Treg的频率明显高于后者.
目的 探討慢性HBV感染者外週血中CD4~+CD25~+Foxp3~+調節性T細胞(RegulatoryT,Treg)的水平,併研究其兩種細胞標誌--CD127分子的低錶達與Foxp3轉錄因子錶達之間的關繫.方法 採集34例免疫耐受期、26例免疫清除期和31例非活動或低(非)複製期慢性HBV感染者的外週全血,用流式細胞術分析CD4~+CD25~+Foxp3~+T細胞和CD4~+CD25~+CD127~(low)T細胞的頻率.結果 在慢性HBV感染者外週血中,免疫耐受組CD4~+CD25~+Foxp3~+T細胞和CD4~+CD25~+CD127~(low)T細胞的頻率均比非活動或低(非)複製組高(Z=-2.693,P=0.007和t=3.251,P=0.002);且病毒暘性組(免疫耐受組+免疫清除組)比非活動或低(非)複製組高(t=2.266,P=0.026和t=3.208,P=0.002);ALT異常組(免疫清除組)與ALT正常組(免疫耐受組+非活動或低(非)複製組)之間的比較差異無統計學意義(P>0.05).91例病例外週血中CD4~+CD25~+Foxp3~+T細胞和CD4~+CD25~+CD127~(low)T細胞之間的錶達有一緻性,但兩者頻率的差異有統計學意義(Z=-4.718,P<0.001).結論 調節性T細胞在慢性HBV感染者病毒高複製組中明顯升高.CD4~+CD25~+T細胞中CD127分子的低錶達與Foxp3轉錄因子的錶達有一緻性,但前者代錶Treg的頻率明顯高于後者.
목적 탐토만성HBV감염자외주혈중CD4~+CD25~+Foxp3~+조절성T세포(RegulatoryT,Treg)적수평,병연구기량충세포표지--CD127분자적저표체여Foxp3전록인자표체지간적관계.방법 채집34례면역내수기、26례면역청제기화31례비활동혹저(비)복제기만성HBV감염자적외주전혈,용류식세포술분석CD4~+CD25~+Foxp3~+T세포화CD4~+CD25~+CD127~(low)T세포적빈솔.결과 재만성HBV감염자외주혈중,면역내수조CD4~+CD25~+Foxp3~+T세포화CD4~+CD25~+CD127~(low)T세포적빈솔균비비활동혹저(비)복제조고(Z=-2.693,P=0.007화t=3.251,P=0.002);차병독양성조(면역내수조+면역청제조)비비활동혹저(비)복제조고(t=2.266,P=0.026화t=3.208,P=0.002);ALT이상조(면역청제조)여ALT정상조(면역내수조+비활동혹저(비)복제조)지간적비교차이무통계학의의(P>0.05).91례병예외주혈중CD4~+CD25~+Foxp3~+T세포화CD4~+CD25~+CD127~(low)T세포지간적표체유일치성,단량자빈솔적차이유통계학의의(Z=-4.718,P<0.001).결론 조절성T세포재만성HBV감염자병독고복제조중명현승고.CD4~+CD25~+T세포중CD127분자적저표체여Foxp3전록인자적표체유일치성,단전자대표Treg적빈솔명현고우후자.
Objective To investigate the level of CD4~+CD25~+Foxp3~+ regulatory T cells and observe relation between expression of Foxp3 and CD127 in peripheral blood of chronic HBV infection. Methods CD4~+CD25~+Foxp3~+and CD4~+CD25~+CD127~(low) Treg in peripheral blood from 34 patients of immune tolerance stage, 26 patients of immune clearance stage and 31 patients of non-active status were quantitatively analyzed by flow cytometry. Results Immune tolerance group presented a higher fraction of CD4~+CD25~+Foxp3~+ and CD4~+CD25~+CD127~(low) Treg than non-active group in chronic HBV infection(Z= -2.693, P=0.007 and t=3.251, P=0.002 ), and HBV positive group also presented a higher fraction than non-active group(t=-2.266,P=0.026 and t=3.208,P=0.002), But ALT normal group is similar to ALT abnormal group(P>0.05). In this study, the relation between expression of CD127~(low) and Foxp3~+ from CD4~+ CD25~+ regulatory T cells was observed, and CD4~+ CD25~+ CD127~(low) Treg presented a higher fraction than CD4~+CD25~+ Foxp3~+ Treg. Conclusion Peripheral Treg in HBV active replication group is higher than HBV negative group of chronic HBV infection. Expression of CD127~(low) is consistent with Foxp3~+ in CD4~+CD25~+ regulatory T cells, but the former is significant higher than the later.
