新疆伊犁地区马和驴博尔纳病病毒自然感染的调查
신강이리지구마화려박이납병병독자연감염적조사
Epidemiological investigation of Borna disease virus infection in horses and donkeys in Yili, Xinjiang
  • 万方数据
    中华微生物学和免疫学杂志 중화미생물학화면역학잡지
    CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
    2009年 4期 321-325 ,共5页

    张英英%展群岭%徐鸣明%余建萍%曾志磊%翟红%刘妍汐%陈晓%彭丹%朱丹%胡永波%霍康%谢鹏

    장영영%전군령%서명명%여건평%증지뢰%적홍%류연석%진효%팽단%주단%호영파%곽강%사붕

    博尔纳病病毒%荧光定量巢式实时逆转录聚合酶链反应%TaqMan探针%伊犁马%伊犁驴 博爾納病病毒%熒光定量巢式實時逆轉錄聚閤酶鏈反應%TaqMan探針%伊犛馬%伊犛驢
    박이납병병독%형광정량소식실시역전록취합매련반응%TaqMan탐침%이리마%이리려
    Borua disease virus%Fluorescence quantitative nested RT-PCR%TaqMan probe%Yilihorses%Yili donkeys
    目的 调查新疆伊犁地区伊犁马和伊犁驴博尔纳病病毒(Boma disease virus,BDV)流行现状,分析BDV种系来源.方法 采用荧光定量巢式实时逆转录聚合酶链反应(fluorescence quan-titative nested RT-PCR,FQ-nRT-PCR),对新疆伊犁地区518匹伊犁马和206头伊犁驴外周血单个核细胞 (peripheral blood mononuclear cell,PBMC)进行BDV p24基因片段检测.对榆测阳性结果 的标本通过BDV p40和质粒标准品的检测进行进一步验证并测序,对其进行基因同源性、氨基酸序列和系统发生分析.结果 5例伊犁马和4例伊犁驴血标本BDV p24榆测阳性,阳性率分别为0.97%和1.94%;9例标本BDV tn0片段检测均阳性,质粒标准品榆测均阴性;BDV p24扩增产物序列与He/80株的同源性为100%.结论 新疆伊犁地区伊犁马和伊犁驴中可能存在BDV的自然感染,该地区BDV流行株与He/80株存在同源性.
    목적 조사신강이리지구이리마화이리려박이납병병독(Boma disease virus,BDV)류행현상,분석BDV충계래원.방법 채용형광정량소식실시역전록취합매련반응(fluorescence quan-titative nested RT-PCR,FQ-nRT-PCR),대신강이리지구518필이리마화206두이리려외주혈단개핵세포 (peripheral blood mononuclear cell,PBMC)진행BDV p24기인편단검측.대유측양성결과 적표본통과BDV p40화질립표준품적검측진행진일보험증병측서,대기진행기인동원성、안기산서렬화계통발생분석.결과 5례이리마화4례이리려혈표본BDV p24유측양성,양성솔분별위0.97%화1.94%;9례표본BDV tn0편단검측균양성,질립표준품유측균음성;BDV p24확증산물서렬여He/80주적동원성위100%.결론 신강이리지구이리마화이리려중가능존재BDV적자연감염,해지구BDV류행주여He/80주존재동원성.
    Objective To investigate the epidemiology of BDV infection in Yili horses and Yili donkeys and to analyze phylogenetic source of BDV in Yili area, Xinjiang. Methods We established fluo- rescence quantitative nested RT-PCR to detect BDV p24 segment in peripheral blood mononuclear cells (PBMCs) of 518 Yili horses and 206 Yili donkeys in Yili area, Xinjiang. Positive products were validated by detecting BDV p40 segment and plasmid to preclude the contamination, and were sequenced to analyze the homology of gene sequence, amino acid sequence and phylogenetic tree. Results The positive rates of BDV infection in PBMCs of 518 Yili horses and 206 Yili donkeys were 0.97% and 1.94%, respectively. The results of BDV p40 segment verification were positive in all of the samples of BDV p24 positive. All the samples tested were not contaminated by plasmid. There was a homology of the gene sequence of positive PCR samples with strain He/80. And the gene sequence revealed more than 93% identical to H1766 and strain V. Conclusion Our study suggested BDV natural infection in Yili horses and Yili donkeys. The en- demic BDV had a high degree of identity to strain He/80.
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