中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2010年
2期
132-135
,共4页
高峰%赵阳%冯永强%霍然%薛文君%王法刚%吕仁荣%薛峰%李强%张健
高峰%趙暘%馮永彊%霍然%薛文君%王法剛%呂仁榮%薛峰%李彊%張健
고봉%조양%풍영강%곽연%설문군%왕법강%려인영%설봉%리강%장건
瘢痕疙瘩%增生性瘢痕%肥大细胞类胰蛋白酶
瘢痕疙瘩%增生性瘢痕%肥大細胞類胰蛋白酶
반흔흘탑%증생성반흔%비대세포류이단백매
Keloids%Hypertrophic scar%Mast cell tryptase
目的 研究肥大细胞类胰蛋白酶(mast cell tryptase,MCT)在病理性瘢痕中的表达及分布情况,探讨MCT基因在瘢痕疙瘩、增生性瘢痕及正常皮肤中是否存在差别.方法 采集未经治疗的瘢痕疙瘩、增生性瘢痕及正常皮肤各20例,应用免疫荧光组化对MCT的表达进行定位,应用实时荧光相对定量PCR进行mRNA基因水平的相对定量分析.结果 MCT主要集中在瘢痕组织的胶原纤维柬之间,以瘢痕组织浅层分布较多;实时荧光PCR相对定量结果显示MCT基因在瘢痕疙瘩中表达高于增生性瘢痕和皮肤(P<0.01),瘢痕疙瘩中MCT基因表达量约为增生性瘢痕的2.5倍,皮肤的5.4倍.结论 MCT在瘢痕的形成中可能起一定的作用.
目的 研究肥大細胞類胰蛋白酶(mast cell tryptase,MCT)在病理性瘢痕中的錶達及分佈情況,探討MCT基因在瘢痕疙瘩、增生性瘢痕及正常皮膚中是否存在差彆.方法 採集未經治療的瘢痕疙瘩、增生性瘢痕及正常皮膚各20例,應用免疫熒光組化對MCT的錶達進行定位,應用實時熒光相對定量PCR進行mRNA基因水平的相對定量分析.結果 MCT主要集中在瘢痕組織的膠原纖維柬之間,以瘢痕組織淺層分佈較多;實時熒光PCR相對定量結果顯示MCT基因在瘢痕疙瘩中錶達高于增生性瘢痕和皮膚(P<0.01),瘢痕疙瘩中MCT基因錶達量約為增生性瘢痕的2.5倍,皮膚的5.4倍.結論 MCT在瘢痕的形成中可能起一定的作用.
목적 연구비대세포류이단백매(mast cell tryptase,MCT)재병이성반흔중적표체급분포정황,탐토MCT기인재반흔흘탑、증생성반흔급정상피부중시부존재차별.방법 채집미경치료적반흔흘탑、증생성반흔급정상피부각20례,응용면역형광조화대MCT적표체진행정위,응용실시형광상대정량PCR진행mRNA기인수평적상대정량분석.결과 MCT주요집중재반흔조직적효원섬유간지간,이반흔조직천층분포교다;실시형광PCR상대정량결과현시MCT기인재반흔흘탑중표체고우증생성반흔화피부(P<0.01),반흔흘탑중MCT기인표체량약위증생성반흔적2.5배,피부적5.4배.결론 MCT재반흔적형성중가능기일정적작용.
Objective To investigate the expression and distribution of mast cell tryptase (MCT) in scar, and to discuss the different MCT gene expression in keloid, hypertrophic scar and normal skin. Methods 20 samples of keloid, 20 samples of hypertrophic scar and 20 samples of normal skin were collected. The distribution of MCT was investigated by immunofluorescence histochemistry, and the MCT mRNA expression was detected by Relative Quantification real-time fluorescent PCR. Results MCT gene was mainly located in the collagen fiber bundles of the scar, especially in the superficial layer of scar. MCT mRNA expression was significantly higher in keloid than that in hypertrophic scar and normal skin (P < 0. 01) . Averagely, the MCT gene expression in keloid was 2. 5 times and S. 4 times of that in hypertrophic scar and normal skin. Conclusions MCT gene may play a role in the pathogenesis of scar.
