哈尔滨医科大学学报
哈爾濱醫科大學學報
합이빈의과대학학보
JOURNAL OF HARBIN MEDICAL UNIVERSITY
2001年
1期
25-26
,共2页
袁友萍%邹小明%杜金荣%聂刚%杨维良
袁友萍%鄒小明%杜金榮%聶剛%楊維良
원우평%추소명%두금영%섭강%양유량
肝移植%ICAM-1%病理学%鼠
肝移植%ICAM-1%病理學%鼠
간이식%ICAM-1%병이학%서
目的 探讨肝移植后再灌注损伤和排斥反应时，肝窦内皮细胞的变化。方法 利用抗肝窦内皮细胞的单克隆抗体对大鼠肝移植后定期采取的肝组织标本染色。结果 对照组(LEW-LEW)于第4天在肝窦壁内可见ICAM-1的强染色，但是昆布氨酸、第Ⅷ因子相关抗原在实验期间内均未见到。高度排斥反应组(ACI-LEW)移植后第1天开始发现ICAM-1的强染色。中度排斥反应组(BN-LEW)移植后第1天开始发现ICAM-1的强染色，移植后1个月开始肝窦壁出现第Ⅷ因子相关抗原。结论 说明慢性排斥反应引起的肝损伤，不仅是以肝动脉为中心的血管损伤，同时也损伤肝窦内皮细胞。
目的 探討肝移植後再灌註損傷和排斥反應時，肝竇內皮細胞的變化。方法 利用抗肝竇內皮細胞的單剋隆抗體對大鼠肝移植後定期採取的肝組織標本染色。結果 對照組(LEW-LEW)于第4天在肝竇壁內可見ICAM-1的彊染色，但是昆佈氨痠、第Ⅷ因子相關抗原在實驗期間內均未見到。高度排斥反應組(ACI-LEW)移植後第1天開始髮現ICAM-1的彊染色。中度排斥反應組(BN-LEW)移植後第1天開始髮現ICAM-1的彊染色，移植後1箇月開始肝竇壁齣現第Ⅷ因子相關抗原。結論 說明慢性排斥反應引起的肝損傷，不僅是以肝動脈為中心的血管損傷，同時也損傷肝竇內皮細胞。
목적 탐토간이식후재관주손상화배척반응시，간두내피세포적변화。방법 이용항간두내피세포적단극륭항체대대서간이식후정기채취적간조직표본염색。결과 대조조(LEW-LEW)우제4천재간두벽내가견ICAM-1적강염색，단시곤포안산、제Ⅷ인자상관항원재실험기간내균미견도。고도배척반응조(ACI-LEW)이식후제1천개시발현ICAM-1적강염색。중도배척반응조(BN-LEW)이식후제1천개시발현ICAM-1적강염색，이식후1개월개시간두벽출현제Ⅷ인자상관항원。결론 설명만성배척반응인기적간손상，불부시이간동맥위중심적혈관손상，동시야손상간두내피세포。
Objective To inquiry pathological changes of endotheliocytes ofhepatic sinusoid while reperfusion and rejection occurred after liver transplantation.Methods We stained liver specimen sampled termly with monoclonal antibodies to endotheliocytes of hepatic sinusoid in rat.Results ICAM-1 was stained markedly in hepatic sinusoid at 4th day,but we didn't find laminine and Ⅷ associated antigen during experiment.Clearly ICAM-1 stained was found in highly rejected group(ACI-LEW)from the first day after transplantation.In the medium group (BN-LEW),we detected that ICAM-1 was stained at 1st day after transplantation,and Ⅷ associated antigen was found in hepatic sinusoid.Conclusion The liver injury resulted from chronic rejection not only destroy hepatic artery but also injure hepatic sinusoid.
