CAJ | 학술논문

目的建立一种简便、快速、准确的检测胃幽门螺杆菌(H.pylori)抗体的新型血清学诊断方法。方法用胶体金标记技术与渗滤法相结合，建立了快速胶体金免疫渗滤法(DIGFA)，与ELISA对比检测145份H.pylori感染血清，并与病理学检查对比检测108份H.pylori血清。做阻断试验和IgG标准品最小蛋白含量的检出观察，对其敏感性、特异性做了分析并调查了216例不同年龄组的人群感染率。结果本法与ELISA相比，两者符合率为95.1％；与病理组织染色检查比较，两者符合率为88.8％。DIGFA可检出IgG标准品的最小蛋白量为10μg·mL-1，阻断试验证明该法检出的确为H.pylori特异抗体。流行病学调查人群感染率为42.5％。结论 DIGFA可作为临床辅助诊断和对消化不良病人的初筛检查，并适用于大规模流行病学调查，具有广阔的应用前景。
목적건립일충간편、쾌속、준학적검측위유문라간균(H.pylori)항체적신형혈청학진단방법。방법용효체금표기기술여삼려법상결합，건립료쾌속효체금면역삼려법(DIGFA)，여ELISA대비검측145빈H.pylori감염혈청，병여병이학검사대비검측108빈H.pylori혈청。주조단시험화IgG표준품최소단백함량적검출관찰，대기민감성、특이성주료분석병조사료216례불동년령조적인군감염솔。결과본법여ELISA상비，량자부합솔위95.1％；여병리조직염색검사비교，량자부합솔위88.8％。DIGFA가검출IgG표준품적최소단백량위10μg·mL-1，조단시험증명해법검출적학위H.pylori특이항체。류행병학조사인군감염솔위42.5％。결론 DIGFA가작위림상보조진단화대소화불량병인적초사검사，병괄용우대규모류행병학조사，구유엄활적응용전경。
Objectives　To establish a simple,quick,accurate serodiagnostic assay for detecting serum antibodies against Helicobacter pylori(H.pylori).Methods　We established quick immunocolloidal gold filtration assay(DIGFA)by combining colloidal gold labelling technique with filtration method.145 sera from cases with H.pylori infection were detected by DIGFA,and the results were compared with that of ELISA and pathological examinations.In addition,blocking test and human IgG protein detection were conducted.The sensitivity,specificity of DIGFA were analysed.Besides,DIGFA was applied to investigate the H.pylori infection situation among persons of different ages.Results　The coincidence rate reached 95.1% compared with the ELISA,and it was 88.8% compared with pathological the examination.The detectable minimum amount of IgG was l0μg/mL.The antibodies detected by DIGFA proved to be specific antibodies by blocking test.The human H.pylori infection rate among 216 persons reached 42.5% by DIGFA.Conclusions　DIGFA can be applied effectively in clinical diagnosis and epidemiological survey.