中成药
中成藥
중성약
CHINESE TRADITIONAL PATENT MEDICINE
2005年
8期
910-913
,共4页
王玉华%魏英勤%王伟%袁久荣
王玉華%魏英勤%王偉%袁久榮
왕옥화%위영근%왕위%원구영
RP-HPLC%芍药苷%白芍%四物汤
RP-HPLC%芍藥苷%白芍%四物湯
RP-HPLC%작약감%백작%사물탕
RP-HPLC%paeoniflorin%Radix Paeoniae Alba%Siwu Decoction (SWD)
目的:建立一种新的测定芍药苷的反相高效液相色谱流动相系统,并令人满意地测定四物汤中芍药苷的含量.方法:色谱条件:Alltima C18柱(250 mm×4.6 mm,5 μm);紫外检测器,检测波长230 nm;乙腈-0.02 mol·L-1磷酸(18:82)为流动相.结果:芍药苷在0.48μg～4.80μg范围内与峰面积积分值呈良好的线性关系,r=0.999 4.平均加样回收率为98.8%,RSD为1.32%(n=5).结论:方法准确,精密,重现性好,结果令人满意.
目的:建立一種新的測定芍藥苷的反相高效液相色譜流動相繫統,併令人滿意地測定四物湯中芍藥苷的含量.方法:色譜條件:Alltima C18柱(250 mm×4.6 mm,5 μm);紫外檢測器,檢測波長230 nm;乙腈-0.02 mol·L-1燐痠(18:82)為流動相.結果:芍藥苷在0.48μg～4.80μg範圍內與峰麵積積分值呈良好的線性關繫,r=0.999 4.平均加樣迴收率為98.8%,RSD為1.32%(n=5).結論:方法準確,精密,重現性好,結果令人滿意.
목적:건립일충신적측정작약감적반상고효액상색보류동상계통,병령인만의지측정사물탕중작약감적함량.방법:색보조건:Alltima C18주(250 mm×4.6 mm,5 μm);자외검측기,검측파장230 nm;을정-0.02 mol·L-1린산(18:82)위류동상.결과:작약감재0.48μg～4.80μg범위내여봉면적적분치정량호적선성관계,r=0.999 4.평균가양회수솔위98.8%,RSD위1.32%(n=5).결론:방법준학,정밀,중현성호,결과령인만의.
AIM: A new RP-HPLC method had been developed and was suitable for the analysis of paeoniflorin in Siwu Decoction (Radix Paeoniae Alba;Radix Rehmaniae Preparata, Radix Angelicae Sinensis;Rhizoma Chuanxion) (SWD). METHODS: Experiment was carried out on Alltima C18 Column and UV detector (230 nm). Acewas a good linear relationship between the concentration of paeoniflorin and area integral value in the range of 0.48-4.80 μg,r =0.999 4. The everage recovery was 98.8% with RSD 1.32% (n =5). CONCLUSION: The method is accurate, precise and reproducible for analysis of paeoniflorin in SWD. The result is satisfactory.
