CAJ | 학술논문

采用特异性引物PCR扩增获得了鲌亚科7属10种鱼类细胞色素b基因的全长序列,初步构建了鲌亚科鱼类的系统发育关系.NJ树和MP树一致表明,鲌亚科鱼类种内所有个体均单独聚群,支持率高;同一属内,除蒙古鲌(Culter mogolicus mongolicus)未能与同属的翘嘴鲌(Culter alburnus)、青梢鲌(Culter dabryi dabryi)单独聚为一支外,团头鲂(Megalobrama amblycephala)与三角鲂(Megalobrama terminal)、翘嘴鲌与青梢鲌均为姐妹种.NJ、MP树显示鲌亚科鱼类属间关系的支持率都较低,鲂属(Megalobrama)与鳊属(Parabramis)间、鲌属(Culter)与原鲌属(Cultrichthys)间、细鳊属(Rasborinus)与半属(Hemiculterella)、属(Hemiculter)间有较近的亲缘关系,在系统演化关系上,细鳊属、半属、依氏鱼属(Ishikauia)较为原始,鲌属与原鲌属、鲂属与鳊属互为姐妹群,为较特化群.
채용특이성인물PCR확증획득료백아과7속10충어류세포색소b기인적전장서렬,초보구건료백아과어류적계통발육관계.NJ수화MP수일치표명,백아과어류충내소유개체균단독취군,지지솔고;동일속내,제몽고백(Culter mogolicus mongolicus)미능여동속적교취백(Culter alburnus)、청소백(Culter dabryi dabryi)단독취위일지외,단두방(Megalobrama amblycephala)여삼각방(Megalobrama terminal)、교취백여청소백균위저매충.NJ、MP수현시백아과어류속간관계적지지솔도교저,방속(Megalobrama)여편속(Parabramis)간、백속(Culter)여원백속(Cultrichthys)간、세편속(Rasborinus)여반속(Hemiculterella)、속(Hemiculter)간유교근적친연관계,재계통연화관계상,세편속、반속、의씨어속(Ishikauia)교위원시,백속여원백속、방속여편속호위저매군,위교특화군.
The full length of cytochrome b gene sequences of 10 species from 7 genus of the Culterinae fishes were amplified by PCR and sequenced using the specific primers. Together with the sequence of Ischikauia steenackeri from GenBank, molecular phylogenetic relationships of the Culterinae fishes were constructed. Both of NJ tree and MP tree showed that individuals within species were clustered with a high bootstrap value; Congenic species, M. amblycephala and M. terminal, C. albumus and C. dabryi dabryi, formed sister relationship except that C. mogolicus mongolicus formed a sister relationship with C. erythropterus. Although the phylogentic relationships within the Culterinae fishes were not strongly supported, a close relationship existed between Megalobrama and Parabramis, Cutter and Cultrichthys, Hemiculter, Hemiculterella and Rasborinus. Evolutionally, Hemiculter, Hemiculterella, Rasborinus andlschikauia were relatively primitive, while Megalobrama and Parabramis, Culler and Cultrichthys were more specialized. These results may be helpful for further understanding of systematic classification and rearrangment of the Culterinae fishes.