CAJ | 학술논문

背景:已有大量的临床和体外实验研究表明,低温热解各向同性碳具有优良的生物学性能,但作为人工关节假体涂层,置入髋关节内的研究还鲜见报道,其在人工半髋关节假体置换中的生物学性能还没有得到验证.目的:通过动物假体置入实验,观察经低温热解各向同性碳(含硅)喷涂后碳质股骨头假体的组织相容性及表面摩擦磨损特性.设计、时间及地点:动物体内进行碳质人工半髋关节替换,随机对照动物实验,于2008-10/2009-04在解放军第二军医大学动物实验中心完成.材料:碳质人工半髋关节由吉林市中心医院提供.股骨头以碳石墨材料为基体,沉积含硅的低温热解各向同性碳为涂层.方法:对16只成年新西兰大白兔进行右侧半髋关节置换术,置入碳质股骨头假体.按照观察时间点随机分为术后6周组4只,术后11周组6只,术后21周组6只,其中21周组从18周开始在动物实验中心草坪上进行诱导性运动,2 h/d.主要观察指标:通过大体、X射线片、组织切片观察假体置入后的组织相容性及界面摩擦磨损现象.结果:碳质股骨头假体在动物体内无毒副作用,无明显炎症反应和异物反应,在碳质假体周围发现有新生软骨组织,运动实验后碳质股骨头表面没有明显磨损和碳颗粒游离现象.结论:涂膜碳质材料作为人工股骨头具有优良的生物相容性和耐磨特能,是一种极具应用前景的人工假体材料.
배경:이유대량적림상화체외실험연구표명,저온열해각향동성탄구유우량적생물학성능,단작위인공관절가체도층,치입관관절내적연구환선견보도,기재인공반관관절가체치환중적생물학성능환몰유득도험증.목적:통과동물가체치입실험,관찰경저온열해각향동성탄(함규)분도후탄질고골두가체적조직상용성급표면마찰마손특성.설계、시간급지점:동물체내진행탄질인공반관관절체환,수궤대조동물실험,우2008-10/2009-04재해방군제이군의대학동물실험중심완성.재료:탄질인공반관관절유길림시중심의원제공.고골두이탄석묵재료위기체,침적함규적저온열해각향동성탄위도층.방법:대16지성년신서란대백토진행우측반관관절치환술,치입탄질고골두가체.안조관찰시간점수궤분위술후6주조4지,술후11주조6지,술후21주조6지,기중21주조종18주개시재동물실험중심초평상진행유도성운동,2 h/d.주요관찰지표:통과대체、X사선편、조직절편관찰가체치입후적조직상용성급계면마찰마손현상.결과:탄질고골두가체재동물체내무독부작용,무명현염증반응화이물반응,재탄질가체주위발현유신생연골조직,운동실험후탄질고골두표면몰유명현마손화탄과립유리현상.결론:도막탄질재료작위인공고골두구유우량적생물상용성화내마특능,시일충겁구응용전경적인공가체재료.
BACKGROUND: Previous experiments demonstrated that low-temperature isotropic pyrolytic carbon has excellent biological features. However, the research regarding low-temperature isotropic pyrolytic carbon prosthesis in hip joints, especially the biological features following hemiarthroplasty are poorly understood. OBJECTIVE: Utilizing implantation experiment, the aim of this study was to investigate the biocompatibility, as well as the interface wear properties of carbon femoral head prosthesis, coated with low-temperature isotropic pyrolytic carbon (silicon). DESIGN, TIME AND SETTING: In vivo hemiarthroplasty, randomized controlled animal experiment. The experiment was performed at the Experimental Animal Center, the Second Military Medical University of Chinese PLA, from October 2008 to April 2009. MATERIALS: Carbon femoral head prostheses.were supplied by Jilin Central Hospital. The carbon femoral head prosthesis was coated with low-temperature isotropic pyrolytic carbon. METHODS: Carbon femoral head prostheses were implanted in 16 adult New Zealand white rabbits. The rabbits were sacrificed at weeks 6 (n=4), 11 (n=6), 21 (n=6), respectively. Rabbits in the 21-week group were induced to move from 18 weeks with 2 hours per day. MAIN OUTCOME MEASURES: The biocompatibility and the interface wear phenomena of the prostheses were investigated by general observation, X-ray observation and tissue slice observation. RESULTS: The carbon femoral head prostheses implanted in animals produced non-toxic side effects, without significant inflammatory response or foreign body reaction. Furthermore, new cartilage tissue around carbon prosthesis was found, while there was no obvious wear debris after sports experiments. CONCLUSION: The film coating carbon materials have excellent biocompatibility and good wear resistance as femoral head prosthesis. Thus, it is a kind of promising biomaterials in prosthesis manufacturing.