CAJ | 학술논문

[目的]利用RAPD RT-PCR的方法研究小麦TP突变体幼苗在光胁迫下基因的表达差异.[方法]以100条随机引物筛选正常光照下小麦TP突变体幼苗和光胁迫下的小麦TP突变体幼苗.[结果]3条引物能扩增出差异性条带,其中TpS23在正常光照下特异性表达,而TpS102和TpS107在光胁迫条件下特异性表达,通过NCBI中的Blastn和Blastx比对发现,TpS23和乌拉尔图小麦的质体Acc1基因同源,TpS102与水稻的Ty3-gypsy反转录转座子同源,而TpS107是一个未知序列.[结论]该研究为克隆这3个基因的全序列和分析这些基因的功能奠定了基础.
[목적]이용RAPD RT-PCR적방법연구소맥TP돌변체유묘재광협박하기인적표체차이.[방법]이100조수궤인물사선정상광조하소맥TP돌변체유묘화광협박하적소맥TP돌변체유묘.[결과]3조인물능확증출차이성조대,기중TpS23재정상광조하특이성표체,이TpS102화TpS107재광협박조건하특이성표체,통과NCBI중적Blastn화Blastx비대발현,TpS23화오랍이도소맥적질체Acc1기인동원,TpS102여수도적Ty3-gypsy반전록전좌자동원,이TpS107시일개미지서렬.[결론]해연구위극륭저3개기인적전서렬화분석저사기인적공능전정료기출.
[Objective]The aim was to study on the differential expression gene in TP mutation of wheat under light stress with RAPD RT-PCR method. [Method]The seedling of TP mutation in wheat under normal light and light stress were screened using 100 random primers. [Result]The results indicated that the expanded products of three primers showed specific character among the normal light line and light stress line, in which, Tps23 expressed only in the normal light line, while, Tps102 and Tps107 specific expressed in the light stress line. Sequence alignments use blastn and blastx showed that Tps23 and Acc1 gene in Triticum urartu shared high similarity in nucleotide level, and the Similarity of amino acid sequence among Tps102 and Ty3-gypsy retrotransposons were 41%. But the Tps107 was an unknown sequence. [Conclusion]This study will provide the basis for clone the full-length and characterize those three genes in TP mutation of wheat.