CAJ | 학술논문

锌指蛋白在调节植物防卫基因表达和抗性反应上起关键作用.目前,对大豆中C3HC4型RING锌指蛋白基因的研究不多.本研究利用核蛋白筛选系统(NTT)筛选大豆(铁丰8号)干旱处理5h的cDNA文库,获得一个RING锌指蛋白基因.该基因全长927bp,编码308个氨基酸,舍有C3HCA-type RING锌指结构域,命名为GmRZFPI.系统进化树分析显示.GmRZFP1属于C3HC4-type锌指亚家族.Real-time PCR结果表明,GmRZFPl基因受干旱、高盐、高温、低温、乙烯和ABA等胁迫诱导表达,表明该蛋白涉及多种胁迫相关的信号传导途径.亚细胞定位结果表明,163hGFP-GmRZFP1融合蛋白定位于细胞核中.本研究结果有助于研究该类基因在大豆逆境应答反应中的作用,阐明大豆抗逆分子机制.
자지단백재조절식물방위기인표체화항성반응상기관건작용.목전,대대두중C3HC4형RING자지단백기인적연구불다.본연구이용핵단백사선계통(NTT)사선대두(철봉8호)간한처리5h적cDNA문고,획득일개RING자지단백기인.해기인전장927bp,편마308개안기산,사유C3HCA-type RING자지결구역,명명위GmRZFPI.계통진화수분석현시.GmRZFP1속우C3HC4-type자지아가족.Real-time PCR결과표명,GmRZFPl기인수간한、고염、고온、저온、을희화ABA등협박유도표체,표명해단백섭급다충협박상관적신호전도도경.아세포정위결과표명,163hGFP-GmRZFP1융합단백정위우세포핵중.본연구결과유조우연구해류기인재대두역경응답반응중적작용,천명대두항역분자궤제.
Zinc-finger proteins play a key role in the regulation expression of defense genes and resistance response of plant.So far,few articles reported C3HC4-type RING zinc-finger protein genes in soybean research.In this study,a RING zinc-finger protein gene was isolated from soybean(Glycine max,Tiefeng 8) cDNA library treated by drought stress for 5h using NTT system.This gene contains 927 bp,coding 308 amino acids,and has a C3HC4-type RING finger domain and named GmRZFP1.Phylogenetic analysis showed that GmRZFP1 belong to C3HC4-type Zinc-finger subfamily.Real-time PCR results showed that the expression of GmRZFP1 gene was induced by drought,high salt,high temperature,low temperature,ethylene and ABA stresses,suggesting that GmRZFP1 might involve in some stresses related signal pathways.Subcellular localization assay indicated that the 163 hGFP-GmRZFP1 fusion protein was located in the nucleus of plant.The results would be useful in the study of functions of this gene family in stress responses of soybean,and clarify molecular mechanisms of stress resistance in soybean.