中国糖尿病杂志
中國糖尿病雜誌
중국당뇨병잡지
CHINESE JOURNAL OF DIABETES
2011年
1期
60-63
,共4页
于虎%马瑞珏%张朔杨%李艳%车永哲%许松山
于虎%馬瑞玨%張朔楊%李豔%車永哲%許鬆山
우호%마서각%장삭양%리염%차영철%허송산
胸腺素β4%糖尿病%损伤愈合%血管内皮生长因子%层粘连蛋白5
胸腺素β4%糖尿病%損傷愈閤%血管內皮生長因子%層粘連蛋白5
흉선소β4%당뇨병%손상유합%혈관내피생장인자%층점련단백5
Thymosin beta 4%Diabetes% Wound healing%Vascular endothelial growth factor% Laminin-5
目的 研究胸腺素β4(Tβ4)调节血管内皮生长因子(VEGF)和层粘连蛋白5(LN-5)的表达,探讨其促进糖尿病大鼠皮肤损伤愈合的机制.方法 60只雄性SD大鼠建立糖尿病大鼠全层皮肤损伤模型(D=8mm),随机分为糖尿病对照(DM)组和Tβ4治疗组[又分为低(L-Tβ4)、中(M-Tβ4)、高(H-Tβ4)3个剂量组].计算皮肤愈合率和表皮迁移率.一侧伤口进行HE染色,弹力纤维、胶原纤维、网状纤维三联染色和VEGF、LN-5免疫组化染色;另一侧伤口进行LN-5 ELISA测定.结果 M-Tβ4和H-Tβ4组各时间点伤口愈合率均较DM组高(P＜0.05或P＜0.01);损伤后10d M-Tβ4和H-Tβ4组的表皮迁移率高于DM组(P＜0.01).Tβ4治疗组肉芽组织中VEGF表达较多、阳性血管较丰富,LN-5在表皮基底细胞和基底膜早期表达;损伤后2d和10d Tβ4治疗组LN-5含量较高,损伤后4d较低.结论 Tβ4可促进糖尿病大鼠皮肤损伤愈合,其机制可能与调节VEGF和LN-5表达有关.
目的 研究胸腺素β4(Tβ4)調節血管內皮生長因子(VEGF)和層粘連蛋白5(LN-5)的錶達,探討其促進糖尿病大鼠皮膚損傷愈閤的機製.方法 60隻雄性SD大鼠建立糖尿病大鼠全層皮膚損傷模型(D=8mm),隨機分為糖尿病對照(DM)組和Tβ4治療組[又分為低(L-Tβ4)、中(M-Tβ4)、高(H-Tβ4)3箇劑量組].計算皮膚愈閤率和錶皮遷移率.一側傷口進行HE染色,彈力纖維、膠原纖維、網狀纖維三聯染色和VEGF、LN-5免疫組化染色;另一側傷口進行LN-5 ELISA測定.結果 M-Tβ4和H-Tβ4組各時間點傷口愈閤率均較DM組高(P＜0.05或P＜0.01);損傷後10d M-Tβ4和H-Tβ4組的錶皮遷移率高于DM組(P＜0.01).Tβ4治療組肉芽組織中VEGF錶達較多、暘性血管較豐富,LN-5在錶皮基底細胞和基底膜早期錶達;損傷後2d和10d Tβ4治療組LN-5含量較高,損傷後4d較低.結論 Tβ4可促進糖尿病大鼠皮膚損傷愈閤,其機製可能與調節VEGF和LN-5錶達有關.
목적 연구흉선소β4(Tβ4)조절혈관내피생장인자(VEGF)화층점련단백5(LN-5)적표체,탐토기촉진당뇨병대서피부손상유합적궤제.방법 60지웅성SD대서건립당뇨병대서전층피부손상모형(D=8mm),수궤분위당뇨병대조(DM)조화Tβ4치료조[우분위저(L-Tβ4)、중(M-Tβ4)、고(H-Tβ4)3개제량조].계산피부유합솔화표피천이솔.일측상구진행HE염색,탄력섬유、효원섬유、망상섬유삼련염색화VEGF、LN-5면역조화염색;령일측상구진행LN-5 ELISA측정.결과 M-Tβ4화H-Tβ4조각시간점상구유합솔균교DM조고(P＜0.05혹P＜0.01);손상후10d M-Tβ4화H-Tβ4조적표피천이솔고우DM조(P＜0.01).Tβ4치료조육아조직중VEGF표체교다、양성혈관교봉부,LN-5재표피기저세포화기저막조기표체;손상후2d화10d Tβ4치료조LN-5함량교고,손상후4d교저.결론 Tβ4가촉진당뇨병대서피부손상유합,기궤제가능여조절VEGF화LN-5표체유관.
ObjectiveTo study the effects of thymosin β4 (Tβ4) on regulation expression of VEGF and LN-5 and on accelerating skin wound healing in diabetic ratMethodsTwo full thickness punch wounds(D=8mm)were made at the costovertebral angle on dorsal surface of each adult male diabetic SD rat weighing 200～250 g.60 diabetic wound models were distributed randomly into the control group (n=15) and the treatment group (n=45), which was subdivided into low(Tβ4 05 μg/50 μl), medium(Tβ4 5 μg/50 μl)and high(Tβ4 15 μg/50 μl)dose groups.The skin wound healing rate and the epidermis migration rate were calculated.Skin sections were applied with H-E stain, trichrome stain and VEGF, LN-5 immunostains.LN-5 concentration was measured by ELISA assay.ResultsCompared with control group, there was a significant increase of wound healing rate in the medium and high dose group at each time point(P<005).Epidermis migration rate of the medium and high dose group was higher than control group(P<001).Immunostain showed there was more VEGF expression and positive blood vessels in the granulation in the treatment group, LN-5 was expressed in the epidermis and the epidermal basement membrane at 2 day.LN-5 ELISA assay showed that LN-5 content was higher in 2 days or 10 days treatment group than in control group,but lower in 4 day group than in than control group.Conclusions Tβ4 may regulate the expression of VEGF and LN-5 and then promotes reepithelization and angiogenesis,finally accelerating skin wound healing in diabetic rat.
