中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2011年
5期
461-464
,共4页
居红格%沈淑萍%耿虹%谢立平%郭建国%蒋剑英%张伟%王丽珍%王欣欣
居紅格%瀋淑萍%耿虹%謝立平%郭建國%蔣劍英%張偉%王麗珍%王訢訢
거홍격%침숙평%경홍%사립평%곽건국%장검영%장위%왕려진%왕흔흔
CD147%基质金属蛋白酶-9%金属蛋白酶抑制物-1%胶质瘤%免疫组化%实时荧光定量RT-PCR
CD147%基質金屬蛋白酶-9%金屬蛋白酶抑製物-1%膠質瘤%免疫組化%實時熒光定量RT-PCR
CD147%기질금속단백매-9%금속단백매억제물-1%효질류%면역조화%실시형광정량RT-PCR
CD147%MMP-9%TIMP-1%Glioma%Immunohistochemistry%Real time PCR
目的 探讨CD147、基质金属蛋白酶(MMP)-9和基质金属蛋白酶抑制物(TIMP)-1在胶质瘤中的表达及其相关性.方法 采用免疫组织化学法对78例石蜡包埋的胶质瘤组织和12例正常脑组织进行标记和分析,应用实时荧光定量RT-PCR方法检测CD147 mRNA的相对含量.结果 CD147、MMP-9、TIMP-1的阳性率分别为62%(48/78)、71%(55/78)、59%(46/78),采用Spearman进行相关性分析,其与胶质瘤的恶性程度均呈正相关(rs=0.2671～0.5631,P＜0.01),CD147与MMP-9呈正相关(rs=0.3576,P＜0.01);CD147、MMP-9、TIMP-1在胶质瘤的临床分级中的表达(低级别组分别为47%、56%和49%;高级别组为80%、89%和71%)差异有统计学意义(x2值分别为9.510、10.702、4.138,P均＜0.05);在胶质瘤的病理分级中,CD147 mRNA相对含量分别为Ⅰ级0.15,Ⅱ级0.27,Ⅲ级0.46,Ⅳ级0.78,随着胶质瘤的级别越高,CD147 mRNA相对含量越高.结论 CD147、MMP-9和TIMP-1可以作为临床判断胶质瘤预后的分子生物学标志.
目的 探討CD147、基質金屬蛋白酶(MMP)-9和基質金屬蛋白酶抑製物(TIMP)-1在膠質瘤中的錶達及其相關性.方法 採用免疫組織化學法對78例石蠟包埋的膠質瘤組織和12例正常腦組織進行標記和分析,應用實時熒光定量RT-PCR方法檢測CD147 mRNA的相對含量.結果 CD147、MMP-9、TIMP-1的暘性率分彆為62%(48/78)、71%(55/78)、59%(46/78),採用Spearman進行相關性分析,其與膠質瘤的噁性程度均呈正相關(rs=0.2671～0.5631,P＜0.01),CD147與MMP-9呈正相關(rs=0.3576,P＜0.01);CD147、MMP-9、TIMP-1在膠質瘤的臨床分級中的錶達(低級彆組分彆為47%、56%和49%;高級彆組為80%、89%和71%)差異有統計學意義(x2值分彆為9.510、10.702、4.138,P均＜0.05);在膠質瘤的病理分級中,CD147 mRNA相對含量分彆為Ⅰ級0.15,Ⅱ級0.27,Ⅲ級0.46,Ⅳ級0.78,隨著膠質瘤的級彆越高,CD147 mRNA相對含量越高.結論 CD147、MMP-9和TIMP-1可以作為臨床判斷膠質瘤預後的分子生物學標誌.
목적 탐토CD147、기질금속단백매(MMP)-9화기질금속단백매억제물(TIMP)-1재효질류중적표체급기상관성.방법 채용면역조직화학법대78례석사포매적효질류조직화12례정상뇌조직진행표기화분석,응용실시형광정량RT-PCR방법검측CD147 mRNA적상대함량.결과 CD147、MMP-9、TIMP-1적양성솔분별위62%(48/78)、71%(55/78)、59%(46/78),채용Spearman진행상관성분석,기여효질류적악성정도균정정상관(rs=0.2671～0.5631,P＜0.01),CD147여MMP-9정정상관(rs=0.3576,P＜0.01);CD147、MMP-9、TIMP-1재효질류적림상분급중적표체(저급별조분별위47%、56%화49%;고급별조위80%、89%화71%)차이유통계학의의(x2치분별위9.510、10.702、4.138,P균＜0.05);재효질류적병리분급중,CD147 mRNA상대함량분별위Ⅰ급0.15,Ⅱ급0.27,Ⅲ급0.46,Ⅳ급0.78,수착효질류적급별월고,CD147 mRNA상대함량월고.결론 CD147、MMP-9화TIMP-1가이작위림상판단효질류예후적분자생물학표지.
Objective To investigate the expressions of CD147,MMP-9 and TIMP-1 in human gliomas and analyze the correlations.Methods Expressions of CD147,MMP-9 and TIMP-1 were assessed in paraffin-embedded specimens collected from 78 gliomas and 12 benign brain lesion tissues by immunohistochemistry.Real time PCR was performed to detect CD147 mRNA expression.Results The positive rates of CD147,MMP-9 and TIMP-1 expression were 62%(48/78),71%(55/78),59%(46/78) respectively.We found a significant positive correlation between CD147,MMP-9,TIMP-1 expressions and poor gliomas differentiation by Spearman analysis(rs=0.2671-0.5631,Ps＜0.01).There was also a significant positive correlation between CD147 and MMP-9 expression(rs =0.3576,P＜0.01).In addition,the expressions of CD147(47% vs.80%,x2=9.510),MMP-9(56% vs.89%,x2=10.702),and TIMP-1(49% vs.71%,x2=4.138) were significantly higher in advanced gilomas than early gliomas(Ps＜0.05).The relative expression levels of CD147 mRNA in gliomas of Ⅰ to Ⅳ pathological grades were 0.15,0.27,0.46,0.78 respectively.Conclusion The expressions of CD147,MMP-9 and TIMP-1 were important characteristic of gliomas,which may serve as biomarkers in the glioma prognostic prediction.
