中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2010年
6期
391-395
,共5页
李琳%张宗敏%刘宇%魏明辉%薛丽燕%邹霜梅%邸雪冰%韩迺珺%张开泰%徐震纲%高燕宁
李琳%張宗敏%劉宇%魏明輝%薛麗燕%鄒霜梅%邸雪冰%韓迺珺%張開泰%徐震綱%高燕寧
리림%장종민%류우%위명휘%설려연%추상매%저설빙%한내군%장개태%서진강%고연저
喉肿瘤%癌,鳞状细胞%肿瘤转移%微RNAs%寡核苷酸序列分析
喉腫瘤%癌,鱗狀細胞%腫瘤轉移%微RNAs%寡覈苷痠序列分析
후종류%암,린상세포%종류전이%미RNAs%과핵감산서렬분석
Laryngeal neoplasms%Carcinoma,squamous cell%Neoplasm metastasis%MicroRNAs%Oligonucleotide array sequence analysis
目的 建立以甲醛固定石蜡包埋组织为材料、基于基因芯片技术的microRNA(miRNA)表达谱的分析方法 ;筛选与喉鳞状细胞癌(简称喉癌)生物学特征密切相关的差异表达miRNA.方法 从喉癌甲醛固定石蜡包埋组织中制备总RNA,经质量鉴定后进行荧光标记.采用Agilent公司的容纳723条人类miRNA探针的基因芯片完成杂交实验,以获得喉癌的miRNA表达谱.以GeneSpring GX和R-Project软件处理分析基因芯片实验数据,筛选与喉癌转移相关的差异表达miRNA.结果 从24例甲醛固定石蜡包埋组织标本中获得了符合基因芯片实验质量标准的RNA样品,并完成了基因芯片杂交及数据分析.从中共鉴定到319个miRNA,有96个miRNA在24例喉癌中均有表达,其中与淋巴结转移密切相关的(检错率<0.05)差异表达miRNA有5个,分别为miR-23a* 、miR-28-5p、miR-15a、miR-16和miR-425.结论 甲醛固定石蜡包埋组织可以提供符合基因芯片分析质量要求的miRNA,是研究miRNA的重要样品资源.从喉癌的miRNA表达谱中筛选出的转移相关差异表达miRNA(miR-23a*、miR-28-5p、miR-15a、miR-16和miR-425)有可能成为评估喉癌转移风险的新型分子标志.
目的 建立以甲醛固定石蠟包埋組織為材料、基于基因芯片技術的microRNA(miRNA)錶達譜的分析方法 ;篩選與喉鱗狀細胞癌(簡稱喉癌)生物學特徵密切相關的差異錶達miRNA.方法 從喉癌甲醛固定石蠟包埋組織中製備總RNA,經質量鑒定後進行熒光標記.採用Agilent公司的容納723條人類miRNA探針的基因芯片完成雜交實驗,以穫得喉癌的miRNA錶達譜.以GeneSpring GX和R-Project軟件處理分析基因芯片實驗數據,篩選與喉癌轉移相關的差異錶達miRNA.結果 從24例甲醛固定石蠟包埋組織標本中穫得瞭符閤基因芯片實驗質量標準的RNA樣品,併完成瞭基因芯片雜交及數據分析.從中共鑒定到319箇miRNA,有96箇miRNA在24例喉癌中均有錶達,其中與淋巴結轉移密切相關的(檢錯率<0.05)差異錶達miRNA有5箇,分彆為miR-23a* 、miR-28-5p、miR-15a、miR-16和miR-425.結論 甲醛固定石蠟包埋組織可以提供符閤基因芯片分析質量要求的miRNA,是研究miRNA的重要樣品資源.從喉癌的miRNA錶達譜中篩選齣的轉移相關差異錶達miRNA(miR-23a*、miR-28-5p、miR-15a、miR-16和miR-425)有可能成為評估喉癌轉移風險的新型分子標誌.
목적 건립이갑철고정석사포매조직위재료、기우기인심편기술적microRNA(miRNA)표체보적분석방법 ;사선여후린상세포암(간칭후암)생물학특정밀절상관적차이표체miRNA.방법 종후암갑철고정석사포매조직중제비총RNA,경질량감정후진행형광표기.채용Agilent공사적용납723조인류miRNA탐침적기인심편완성잡교실험,이획득후암적miRNA표체보.이GeneSpring GX화R-Project연건처리분석기인심편실험수거,사선여후암전이상관적차이표체miRNA.결과 종24례갑철고정석사포매조직표본중획득료부합기인심편실험질량표준적RNA양품,병완성료기인심편잡교급수거분석.종중공감정도319개miRNA,유96개miRNA재24례후암중균유표체,기중여림파결전이밀절상관적(검착솔<0.05)차이표체miRNA유5개,분별위miR-23a* 、miR-28-5p、miR-15a、miR-16화miR-425.결론 갑철고정석사포매조직가이제공부합기인심편분석질량요구적miRNA,시연구miRNA적중요양품자원.종후암적miRNA표체보중사선출적전이상관차이표체miRNA(miR-23a*、miR-28-5p、miR-15a、miR-16화miR-425)유가능성위평고후암전이풍험적신형분자표지.
Objective To establish DNA microarrays-based microRNA (miRNA) expression profiles of squamous cell carcinoma of larynx, using archived formalin-fixed paraffin-embedded tissue blocks,and to screen out and identify the differentially expressed miRNAs associated with the biological characteristics of this malignant disease. Methods Total RNA was prepared from the formalin-fixed paraffin-embedded tissue blocks. After quality identification and fluorescent labeling, the RNA samples were hybridized with the Agilent human miRNA microarrays which contains 723 probes for human miRNAs. The data was processed with the softwares GeneSpring GX and R-Project. Results From the formalin-fixed paraffin-embedded tumor blocks collected, 24 RNA samples were obtained with the quality accorded to the requirement of miRNA microarray analysis, and both the hybridization and consequent data processing were accomplished. A total of 319 miRNAs were identified and among them 96 were detected in all the 24 formalin-fixed paraffin-embedded blocks of laryngeal carcinoma;and 5 differentially expressed miRNAs (false discovery rate < 0. 05) were found to be associated significantly with the lymphatic metastasis of laryngeal squamous cell carcinoma (P <0. 05), including miR-23a* , miR-28-5p, miR-15a, miR-16 and miR-425. Conclusions Histopathlogical archives of well-annotated formalin- fixed paraffin-embedded tissue specimens are the valuable resources for miRNA study including to collect RNA samples for miRNA microarray analysis. A panel of differentially expressed miRNAs (miR-23a* , miR-28-5p, miR-15a, miR-16 and miR-425) derived from the miRNA expression profile may serve as the potential molecular biomarkers for the prediction of metastasis development in laryngeal squamous cell carcinoma.
