CAJ | 학술논문

探讨腺病毒介导的胰岛素样生长因子Ⅰ(Ad-IGF-Ⅰ)对NOD鼠胰岛炎及胰岛β细胞凋亡的影响.结果发现Ad-IGF-Ⅰ可减少糖尿病的累积发病率,降低胰岛炎积分、促进胰岛β细胞增殖,减少β细胞凋亡率,使Fas、半胱氨酸天冬氨酸蛋白酶3(caspase-3)的蛋白低表达,Bcl-xl蛋白高表达.提示Ad-IGFⅠ可减少NOD鼠胰岛炎,通过Fas和Bcl-xl途径发挥抗凋亡作用.
탐토선병독개도적이도소양생장인자Ⅰ(Ad-IGF-Ⅰ)대NOD서이도염급이도β세포조망적영향.결과 발현Ad-IGF-Ⅰ가감소당뇨병적루적발병솔,강저이도염적분、촉진이도β세포증식,감소β세포조망솔,사Fas、반광안산천동안산단백매3(caspase-3)적단백저표체,Bcl-xl단백고표체.제시Ad-IGFⅠ가감소NOD서이도염,통과Fas화Bcl-xl도경발휘항조망작용.
To explore the protective effect of adenovirus mediated IGF-Ⅰgene(Ad-IGF-Ⅰ)transfer on non-obese diabetic(NOD)mice. The results showed that the incidence of diabetes and degree of insulitis were significantly reduced in mice receiving Ad-IGF- Ⅰ . Treatment with Ad-IGF- Ⅰ significantly decreased apoptosis rate,expression of Fas and caspase-3, and increased expression of Bcl-xl. This study indicates the potential of IGF- Ⅰ gene therapy in protecting NOD mice from insulitis and apoptosis.