CAJ | 학술논문

目的探讨苦参碱应用前后C6脑胶质瘤大鼠模型中Fas因子的表达变化及意义.方法采用脑立体定向技术,将体外培养的C6胶质瘤细胞注入大鼠尾状核区制备胶质瘤大鼠模型,并根据是否用药及用药量的多少分为空白对照组、冰片组、苦参碱低剂量组、苦参碱高剂量组、苦参碱低剂量+冰片组、苦参碱高剂量+冰片组.通过大鼠生存状态、标本的大体所见、MRI、HE染色观察苦参碱对胶质瘤大鼠模型生存质量及胶质瘤体积的影响,用免疫组织化学方法检测苦参碱对胶质瘤大鼠模型肿瘤细胞中Fas表达的影响.结果大鼠生存状态、标本的大体所见、MRI及HE染色显示苦参碱可显著提高胶质瘤大鼠模型的生存质量,抑制胶质瘤细胞增殖.免疫组化结果显示,苦参碱低剂量+冰片组(98.16±11.82)、苦参碱高剂量+冰片组(1 12.80±12.12)Fas表达高于空白对照组(39.09±7.79)、冰片组(46.87±7.43)、苦参碱低剂量组(42.41±7.83)、苦参碱高剂量组(44.20±7.47),苦参碱高剂量+冰片组Fas表达高于苦参碱低剂量+冰片组,差异均有统计学意义(P＜0.05).结论苦参碱能增加胶质瘤细胞中Fas的表达,抑制胶质瘤细胞增殖.
목적 탐토고삼감응용전후C6뇌효질류대서모형중Fas인자적표체변화급의의.방법채용뇌입체정향기술,장체외배양적C6효질류세포주입대서미상핵구제비효질류대서모형,병근거시부용약급용약량적다소분위공백대조조、빙편조、고삼감저제량조、고삼감고제량조、고삼감저제량+빙편조、고삼감고제량+빙편조.통과대서생존상태、표본적대체소견、MRI、HE염색관찰고삼감대효질류대서모형생존질량급효질류체적적영향,용면역조직화학방법검측고삼감대효질류대서모형종류세포중Fas표체적영향.결과 대서생존상태、표본적대체소견、MRI급HE염색현시고삼감가현저제고효질류대서모형적생존질량,억제효질류세포증식.면역조화결과현시,고삼감저제량+빙편조(98.16±11.82)、고삼감고제량+빙편조(1 12.80±12.12)Fas표체고우공백대조조(39.09±7.79)、빙편조(46.87±7.43)、고삼감저제량조(42.41±7.83)、고삼감고제량조(44.20±7.47),고삼감고제량+빙편조Fas표체고우고삼감저제량+빙편조,차이균유통계학의의(P＜0.05).결론 고삼감능증가효질류세포중Fas적표체,억제효질류세포증식.
Objective To investigate the effect of matrine on Fas expression in C6 glima in a tumor-bearing rat model. Methods Cultured cerebral glioma C6 cells wgre injected stereotactically into the lef tcaudate nucleus of the rats.The ratswere randomized into untreated group,bomeol-treated group,low-dose matrine group,high-dose maaine group,low-dose matrine+bomeol group,and high-dose matrine+borneol group.The effect of matrine on the quality of life of the rats and the glioma volume was evaluated according to the survival state of the rats and by gross observation,magnetic resonance imaging(MRJ)and HE smining of the brain tissue.Immunohistochemistry was performed to detect Fas expression in the glioma cells. Results The survival state ofthe rats,gross observation of the brain specimen. and results of MRI and HE staining all showed that matrine significantly improved the quality oflife of the glioma-bearing rats and inhibited the glioma cell proliferation.Fas expression Was significantly higher in low-dose matrine+bomeol group(98.16±11.82) and high-dose matrine+bomeol group(112.80±12.12)than in untreated group(39.09±7.79),bomeol group(46.87±7.43),low-dose matrinc group(42.41±7.83),and high-dose matrine group(44.20±7.47)(P＜0.05).Fas expression Was obviously upregulated in the high-dose matrine+bomeol group aS compared with the low-dose matrine+bomeol group(P＜0.05).Conclusion Matrine Can significantly upregulate Fas expression in glioma and inhibit glioma cell proliferation in the glioma-bearing rat model.