CAJ | 학술논문

目的探讨人巨细胞病毒(human eytomegalovirus,HCMV)UL143序列在临床患儿低传代分离株中的多态性及其与临床疾病的关系.方法对19株HCMV临床低传代分离株进行HCMV-UL143 PCR扩增分析及伞序列测定分析.结果 19株HCMV感染患儿临床分离株均因碱基插入造成移码突变,开放阅读框架(ORF)比Toledo株短.根据序列变异情况可将19个序列分为2组,第1组16个序列新增一个MYRISTYL位点;缺失2个PKC磷酸化位点.未发现黄疸、小头畸形、先天性巨结肠等不同疾病类型的序列之间的差异.结论 HCMV-UL143较多存在于临床低传代分离株中,序列呈现一定多态性.
목적 탐토인거세포병독(human eytomegalovirus,HCMV)UL143서렬재림상환인저전대분리주중적다태성급기여림상질병적관계.방법 대19주HCMV림상저전대분리주진행HCMV-UL143 PCR확증분석급산서렬측정분석.결과 19주HCMV감염환인림상분리주균인감기삽입조성이마돌변,개방열독광가(ORF)비Toledo주단.근거서렬변이정황가장19개서렬분위2조,제1조16개서렬신증일개MYRISTYL위점;결실2개PKC린산화위점.미발현황달、소두기형、선천성거결장등불동질병류형적서렬지간적차이.결론 HCMV-UL143교다존재우림상저전대분리주중,서렬정현일정다태성.
Objective To explore the relationship between ULI43 sequence variability and clini-cal disease. Methods UL143 from samples obtained from suspected congenitally human cytomegalovirus (HCMV) infected symptomatic infants were PCR amplified and sequenced. Results There were not too much sequence variability of UL143 compared with Toledo. But no one was completely identical to Toledo, and all UL143 ORFs were shorter than Toledo for frame-shift. Conclusion HCMV-UL143 existed in moat of low passage isolates and sequences were variable. No obvious linkage was observed between UL143 poly-morphisms and outcome of suspected congenital HCMV infection.