CAJ | 학술논문

目的观察survivin基因mRNA及蛋白在鼻咽癌的表达及其与临床的关系.方法采用原位杂交和免疫组织化学技术,分别检测survivin基因及蛋白的表达情况.结果在64例鼻咽癌中,survivin基因mRNA阳性表达42例(65.6%),其中高表达30例(46.9%);在有详尽诊疗资料的22例鼻咽癌中,Ⅲ+Ⅳ期阳性表达率为66.7%,高于Ⅰ+Ⅱ期的50.0%.Survivin蛋白阳性表达46例(71.9%),其中高表达38例(59.4%);Ⅲ+Ⅳ期阳性表达率为61.1%,高于Ⅰ+Ⅱ期的50.0%.Survivin基因表达与鼻咽癌患者的年龄、性别无相关性(P＞0.05).30例慢性鼻咽炎患者鼻咽部组织survivin基因mRNA及蛋白阳性表达分别为10例(33.3%)及7例(23.3%),均低于鼻咽癌组,差异均有统计学意义(γ2=12.04和γ2=19.57,均P＜0.01).64例鼻咽癌中survivin基因mRNA及蛋白共同表达阳性者36例(56.2%),经相关检验它们的表达呈正相关(关联系数(4)=0.43).结论 Survivin基因表达可能在鼻咽癌发生、发展过程中起一定作用,检测survivin基因mRNA和蛋白在鼻咽癌中的表达对鼻咽癌的诊断、临床分期和预后有参考的价值.
목적 관찰survivin기인mRNA급단백재비인암적표체급기여림상적관계.방법 채용원위잡교화면역조직화학기술,분별검측survivin기인급단백적표체정황.결과 재64례비인암중,survivin기인mRNA양성표체42례(65.6%),기중고표체30례(46.9%);재유상진진료자료적22례비인암중,Ⅲ+Ⅳ기양성표체솔위66.7%,고우Ⅰ+Ⅱ기적50.0%.Survivin단백양성표체46례(71.9%),기중고표체38례(59.4%);Ⅲ+Ⅳ기양성표체솔위61.1%,고우Ⅰ+Ⅱ기적50.0%.Survivin기인표체여비인암환자적년령、성별무상관성(P＞0.05).30례만성비인염환자비인부조직survivin기인mRNA급단백양성표체분별위10례(33.3%)급7례(23.3%),균저우비인암조,차이균유통계학의의(γ2=12.04화γ2=19.57,균P＜0.01).64례비인암중survivin기인mRNA급단백공동표체양성자36례(56.2%),경상관검험타문적표체정정상관(관련계수(4)=0.43).결론 Survivin기인표체가능재비인암발생、발전과정중기일정작용,검측survivin기인mRNA화단백재비인암중적표체대비인암적진단、림상분기화예후유삼고적개치.
Objective To investigate the relationship between survivin mRNA and protein expression and nasopharyngeal carcinoma(NPC).Methods Survivin mRNA and protein were detected by in situ hybridization and immunohistochemical S-P staining respectively.Results Among 64 cases of NPC,42 cases(65.6%)were positive for survivin mRNA expression,30 cafes(46.9%)had high expression level.In 46 cases(71.9%)of NPC were positivefor survivin protein expression,and 38 cases(59.4%)had high expression level.In 22 cases of NPC with detailed clinical information,the positive expression rates of survivin mRNA and protein in stagesⅢ+Ⅳ of NPC were 66.7%and61.1%respectively,which were higher than those in stages Ⅰ+Ⅱof NPC(50.0%and 50.0%respectively).Therewas no significant difference between survivin mRNA and protein expression regarding age or gender of NPC patients(all P＞0.05).The positive expression rates of survivin mRNA and protein in chronic nasopharyngitis group were 33.3%and 23.3%respectively,which were lower than those in NPC group(x2=12.04,P＜0.01 and x2=19.57,P＜0.01,respectively).In 64 cases of NPC,36 cases were positive for both survivin mRNA and protein,and the expression of survivin mRNA and protein showed positive correlation((4)=0.43).Conclusion The expression of survivin gene may play some roles in the pathogenesis of NPC.Detection of survivin mRNA and protein will be helpful for diagnosis,clinical staging and prognosis of NPC.