中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2008年
2期
136-140
,共5页
白细胞介素6%钙感应性受体%遗传多态性%骨量
白細胞介素6%鈣感應性受體%遺傳多態性%骨量
백세포개소6%개감응성수체%유전다태성%골량
interleukin-6%calcium sensing receptor%genetic polymorphism%bone mass
目的 探讨白细胞介素6(interleukin-6,IL-6)与钙感应性受体(calcium sensing receptor,CASR)基因多态性对青春期女童骨量增长的交互作用.方法 选择228名9-11岁半未月经初潮的健康女童进行两年追踪,采用双能X线骨密度仪检测对象追踪前后全身、左侧近端股骨(包括股骨颈、大转子、粗隆间和华氏三角区)和L1-L4腰椎骨矿含量(bone mineral content,BMC)和骨密度(bone mineral density,BMD),采用聚合酶链反应-限制性片段长度多态性技术检测IL-6-634C/G位点多态性,等位基因特异性突变分离扩增-PCR技术检测CASR A986S位点多态性.结果 176名女童完成整个研究.IL-6基因-634C/G和CASR基因A986S位点多态性与青春期女童骨量增长有关联,IL-6基因-634C/G位点CG+GG基因型女童全身和股骨大转子BMD较CC基因型分别低25.7%和20.6%,CASR基因A986S位点AS+SS基因型女童L1.L4腰椎BMC和华氏三角区BMD增长率较AA基因型分别低14.9%和51.3%,差异有统计学意义(P＜0.05).交互作用分析发现,同时具有IL-6基因-634C/G位点G等位基因和CASR基因A986S位点S等位基因的女童,其股骨颈和L1-L4腰椎BMC增长率最低.结论 同时具有IL-6基因-634C/G位点G等位基因和CASR基因A986S位点S等位基因的女童,可能是低骨量增长的危险人群.
目的 探討白細胞介素6(interleukin-6,IL-6)與鈣感應性受體(calcium sensing receptor,CASR)基因多態性對青春期女童骨量增長的交互作用.方法 選擇228名9-11歲半未月經初潮的健康女童進行兩年追蹤,採用雙能X線骨密度儀檢測對象追蹤前後全身、左側近耑股骨(包括股骨頸、大轉子、粗隆間和華氏三角區)和L1-L4腰椎骨礦含量(bone mineral content,BMC)和骨密度(bone mineral density,BMD),採用聚閤酶鏈反應-限製性片段長度多態性技術檢測IL-6-634C/G位點多態性,等位基因特異性突變分離擴增-PCR技術檢測CASR A986S位點多態性.結果 176名女童完成整箇研究.IL-6基因-634C/G和CASR基因A986S位點多態性與青春期女童骨量增長有關聯,IL-6基因-634C/G位點CG+GG基因型女童全身和股骨大轉子BMD較CC基因型分彆低25.7%和20.6%,CASR基因A986S位點AS+SS基因型女童L1.L4腰椎BMC和華氏三角區BMD增長率較AA基因型分彆低14.9%和51.3%,差異有統計學意義(P＜0.05).交互作用分析髮現,同時具有IL-6基因-634C/G位點G等位基因和CASR基因A986S位點S等位基因的女童,其股骨頸和L1-L4腰椎BMC增長率最低.結論 同時具有IL-6基因-634C/G位點G等位基因和CASR基因A986S位點S等位基因的女童,可能是低骨量增長的危險人群.
목적 탐토백세포개소6(interleukin-6,IL-6)여개감응성수체(calcium sensing receptor,CASR)기인다태성대청춘기녀동골량증장적교호작용.방법 선택228명9-11세반미월경초조적건강녀동진행량년추종,채용쌍능X선골밀도의검측대상추종전후전신、좌측근단고골(포괄고골경、대전자、조륭간화화씨삼각구)화L1-L4요추골광함량(bone mineral content,BMC)화골밀도(bone mineral density,BMD),채용취합매련반응-한제성편단장도다태성기술검측IL-6-634C/G위점다태성,등위기인특이성돌변분리확증-PCR기술검측CASR A986S위점다태성.결과 176명녀동완성정개연구.IL-6기인-634C/G화CASR기인A986S위점다태성여청춘기녀동골량증장유관련,IL-6기인-634C/G위점CG+GG기인형녀동전신화고골대전자BMD교CC기인형분별저25.7%화20.6%,CASR기인A986S위점AS+SS기인형녀동L1.L4요추BMC화화씨삼각구BMD증장솔교AA기인형분별저14.9%화51.3%,차이유통계학의의(P＜0.05).교호작용분석발현,동시구유IL-6기인-634C/G위점G등위기인화CASR기인A986S위점S등위기인적녀동,기고골경화L1-L4요추BMC증장솔최저.결론 동시구유IL-6기인-634C/G위점G등위기인화CASR기인A986S위점S등위기인적녀동,가능시저골량증장적위험인군.
Objective To investigate the effect of interleukin-6(IL-6)and calcium sensing receptor(CASR)gene polymorphisms on bone mass accrual in Chinese adolescent girls.Methods A total of 228 premenarche Chinese girls(9-11.5 years)were recruited for a 2-year study.Bone mineral densities(BMD)and bone mineral contents(BMC)in the total body,total left hip including femoral neck,trochanter,intertrochanter and Ward's triangle area,and lumbar spine(L1-L4)were measired by DEXA.The-634C/G polymorphism of IL-6 gene was detected by PCR-restriction fragment length polymorphism and A986S polymorphism of CASR gene was detected by allele-special mutagenically separated amplication-PCR.Results There were 176 available subjects when the 2-year study was completed.The-634C/G polymorphism of IL-6 gene and A986S polymorphism of CASR gene were significantly associated with bone mass accrual after adjusting the potential confounding factors.Girls with CC genotype of IL-6-634G/C gene had higher percentage of increase in BMD of total body(P=0.027)and femoral trochanter(P=0.028)than those with CG+GG genotypes.Girls with AA genotype of CASR gene had greater percentage of increase in L1-L4 lumbar spine BMC(P=0.022)and Ward's triangle area BMD(P=0.049)than their AS+SS counterparts.Subjects with G allele of IL-6 gene in combination with S allele of CASR gene had less increase in BMC of femoral neck and L1-L4 lumbar spine.Conclusion Adolescent girls carrying the G allele of IL-6 gene at-634C/G and S allele of CASR gene at A986S could potentially be the risk population of lower bone mass accrual.
