中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2011年
5期
322-325
,共4页
程海%曾令宇%潘彬%宋国梁%田静%陈种%闫志凌%李振宇%徐开林
程海%曾令宇%潘彬%宋國樑%田靜%陳種%閆誌凌%李振宇%徐開林
정해%증령우%반빈%송국량%전정%진충%염지릉%리진우%서개림
造血干细胞移植%移植物抗宿主病%Th17细胞
造血榦細胞移植%移植物抗宿主病%Th17細胞
조혈간세포이식%이식물항숙주병%Th17세포
Stem cell transplantation%Graft host versus disease%Th17 cells
目的 探讨Th17细胞在急性移植物抗宿主病(aGVHD)发病早期的作用及机制.方法 以C57/B6为供鼠,BABL/c为受鼠,致死性全身照射(TBI)后输注供鼠骨髓细胞和脾细胞混合细胞悬液,建立小鼠aGVHD模型.实验分为4组:正常对照组;单纯照射组(IRD);异基因骨髓移植(alloBMT)+二甲基亚砜(DMSO)组;allo-BMT+常山酮(HF)组.从-1 d开始到+10 d,观察小鼠弓背、翘毛、体质量变化等aGVHD的表现并进行临床评分.动态观察小鼠aGVHD发生及存活情况.取小鼠的肝、小肠和皮肤进行病理学分析.用流式细胞术检测移植后小鼠Th1/Th17亚群的变化.结果 移植后实验组小鼠均发生aGVHD,allo-BMT+HF组小鼠aGVHD表现较allo-BMT+DMSO组小鼠严重.病理学分析示allo-BMT+HF组小鼠皮肤损伤较轻,而肝脏和小肠损伤较重,与allo-BMT+DMSO组比较,+6 d allo-BMT+HF组Th17细胞比例明显下降(分别为1.04%和0.71%),Th1比例明显升高(分别为5.35%和12.81%),Th1/Th17比值显著升高(P<0.05).结论 异基因造血干细胞移植早期阻断Th17导致Th1比例明显升高(5.31和18.07),加重aGVHD.
目的 探討Th17細胞在急性移植物抗宿主病(aGVHD)髮病早期的作用及機製.方法 以C57/B6為供鼠,BABL/c為受鼠,緻死性全身照射(TBI)後輸註供鼠骨髓細胞和脾細胞混閤細胞懸液,建立小鼠aGVHD模型.實驗分為4組:正常對照組;單純照射組(IRD);異基因骨髓移植(alloBMT)+二甲基亞砜(DMSO)組;allo-BMT+常山酮(HF)組.從-1 d開始到+10 d,觀察小鼠弓揹、翹毛、體質量變化等aGVHD的錶現併進行臨床評分.動態觀察小鼠aGVHD髮生及存活情況.取小鼠的肝、小腸和皮膚進行病理學分析.用流式細胞術檢測移植後小鼠Th1/Th17亞群的變化.結果 移植後實驗組小鼠均髮生aGVHD,allo-BMT+HF組小鼠aGVHD錶現較allo-BMT+DMSO組小鼠嚴重.病理學分析示allo-BMT+HF組小鼠皮膚損傷較輕,而肝髒和小腸損傷較重,與allo-BMT+DMSO組比較,+6 d allo-BMT+HF組Th17細胞比例明顯下降(分彆為1.04%和0.71%),Th1比例明顯升高(分彆為5.35%和12.81%),Th1/Th17比值顯著升高(P<0.05).結論 異基因造血榦細胞移植早期阻斷Th17導緻Th1比例明顯升高(5.31和18.07),加重aGVHD.
목적 탐토Th17세포재급성이식물항숙주병(aGVHD)발병조기적작용급궤제.방법 이C57/B6위공서,BABL/c위수서,치사성전신조사(TBI)후수주공서골수세포화비세포혼합세포현액,건립소서aGVHD모형.실험분위4조:정상대조조;단순조사조(IRD);이기인골수이식(alloBMT)+이갑기아풍(DMSO)조;allo-BMT+상산동(HF)조.종-1 d개시도+10 d,관찰소서궁배、교모、체질량변화등aGVHD적표현병진행림상평분.동태관찰소서aGVHD발생급존활정황.취소서적간、소장화피부진행병이학분석.용류식세포술검측이식후소서Th1/Th17아군적변화.결과 이식후실험조소서균발생aGVHD,allo-BMT+HF조소서aGVHD표현교allo-BMT+DMSO조소서엄중.병이학분석시allo-BMT+HF조소서피부손상교경,이간장화소장손상교중,여allo-BMT+DMSO조비교,+6 d allo-BMT+HF조Th17세포비례명현하강(분별위1.04%화0.71%),Th1비례명현승고(분별위5.35%화12.81%),Th1/Th17비치현저승고(P<0.05).결론 이기인조혈간세포이식조기조단Th17도치Th1비례명현승고(5.31화18.07),가중aGVHD.
Objective To explore the role of Th17 cells in early onset of acute graft-versus-host disease(aGVHD) and its mechanism. Methods Mice aGVHD model was established by irradiated BABL/c mice inoculated with mixed suspension of C57BL/6 bone marrow cells and splenocytes. The mice were divided into 4 groups:①normal control,②irradiated group, ③allo-BMT + DMSO group,④allo-BMT + halofugine (HF) group. HF was given intraperitoneally at 5 μg per mouse from -1 d to + 10 d after allogeneic bone marrow transplantation(allo-BMT). Mice aGVHD symptoms and survival were observed. Th1/Th17 cells ratio was evaluated by flow cytometry. Results All the experimental groups ③ and ④developed aGVHD after transplantation. More severe aGVHD was observed in group ④than in group ③. HF prevented cutaneous aGVHD in all the mice, but augmented hepatic and small intestine GVHD. The percentage of Th17 cells and the ratio of Th1/Th17 were significantly higher while the percentage of Th1 cells was significantly lower in group ④ at day+6(P<0.05). Conclusion Early blockage of Th17 cell results in increase of Th1 cell percentage, which exacerbates aGVHD.
