精细化工
精細化工
정세화공
FINE CHEMICALS
2001年
5期
302-304
,共3页
金勇%徐社阳%刘宗惠%魏德卿
金勇%徐社暘%劉宗惠%魏德卿
금용%서사양%류종혜%위덕경
猪皮%提取%胶原
豬皮%提取%膠原
저피%제취%효원
对以新鲜猪皮为原料,采用氯化钠/三羟甲基氨基甲烷-盐酸缓冲液(pH=7.5)为萃取液以不同浓度(0.45 mol/L及1.00 mol/L)分两次萃取,用冰醋酸/氯化钠沉淀,然后用冰醋酸溶解精制纯化后再中和提取胶原的新方法进行了研究。所提取胶原的纯度以羟脯氨酸质量分数为基准,采用分光光度法测定为95.7%。其结构为红外光谱所确证。
對以新鮮豬皮為原料,採用氯化鈉/三羥甲基氨基甲烷-鹽痠緩遲液(pH=7.5)為萃取液以不同濃度(0.45 mol/L及1.00 mol/L)分兩次萃取,用冰醋痠/氯化鈉沉澱,然後用冰醋痠溶解精製純化後再中和提取膠原的新方法進行瞭研究。所提取膠原的純度以羥脯氨痠質量分數為基準,採用分光光度法測定為95.7%。其結構為紅外光譜所確證。
대이신선저피위원료,채용록화납/삼간갑기안기갑완-염산완충액(pH=7.5)위췌취액이불동농도(0.45 mol/L급1.00 mol/L)분량차췌취,용빙작산/록화납침정,연후용빙작산용해정제순화후재중화제취효원적신방법진행료연구。소제취효원적순도이간포안산질량분수위기준,채용분광광도법측정위95.7%。기결구위홍외광보소학증。
In this paper,a new technology for extraction of collagen from pig skin was studied.NaCl/Tris-HCl buffer(pH=7.5) was used as extractant to extract collagen two times,firstly using 0.45 mol/L and secondly 1.00 mol/L NaCl/Tris-HCl buffer.Then NaCl/HAc solution was used as precipitant and HAc as solvent to refine collagen.The purity of collagen was 95.7% based on the hydroxyproline content determined by UV.The struture of collagen was identified by FTIR.