CAJ | 학술논문

利用量子点良好的光谱特征和光化学稳定性, 结合免疫分析技术, 对心肌肌钙蛋白I(cTnI)特异性进行定量检测. 用量子点标记cTnI的单克隆抗体(2F11), 通过SDS-PAGE电泳证明标记成功. 斑点免疫膜渗滤法证明标记后的2F11仍具有良好的生物学活性, 再将标记并纯化后的2F11与NC膜上不同浓度的cTnI进行免疫反应, 使用ImageMaster图像分析软件对膜上荧光斑点图像进行定量分析. 应用此方法测得cTnI的浓度和斑点处相对荧光值有良好的线性关系(R~2=0.9966), 最低检出值为120 ng.
이용양자점량호적광보특정화광화학은정성, 결합면역분석기술, 대심기기개단백I(cTnI)특이성진행정량검측. 용양자점표기cTnI적단극륭항체(2F11), 통과SDS-PAGE전영증명표기성공. 반점면역막삼려법증명표기후적2F11잉구유량호적생물학활성, 재장표기병순화후적2F11여NC막상불동농도적cTnI진행면역반응, 사용ImageMaster도상분석연건대막상형광반점도상진행정량분석. 응용차방법측득cTnI적농도화반점처상대형광치유량호적선성관계(R~2=0.9966), 최저검출치위120 ng.
Quantum dots have physical and optical properties that make them useful tools for high-resolution labeling immunoassay. In this work, a rapid and simple method of quantitative immunoassay for Cardiac tropo-nin I(cTnI) was developed with quantum dots-labeled antibodies. The monoclonal antibodies of cTnI(2F11) could be labeled with CdTe quantum dots and the coupled product (CdTe-2F11) were characterized by SDS-PAGE. The result of immunofiltration assay indicats that the CdTe-2Fl 1 maintains the antibody activity. The cTnI at the different concentrations in NC membrane could react with CdTe-2F11 and be detected with ImageMaster to analyze the fluorescence intensity of the immunodotting. The results show that the detection limit of cTnI is 120 ng, and there is a good linear relation between concentration of cTnI and the fluorescence intensity(R~2 =0.9966).