CAJ | 학술논문

以龙眼(Dimocarpus longan Lour.CV.Longyou)正常成花花芽和成花逆转花芽为材料,应用cDNA-AFLP技术.通过64对引物组合的cDNA-AFLP分析获得了2 560条扩增片段,选择有明显差异的片段进行回收和测序,获得了14条核苷酸序列,其中有13个片段在GenBank数据库中发现同源序列,1个功能未知.通过核酸和氨基酸比对分析,112基因片段同源于银灰杨(Populustremula × Populus alba)中编码Nek激酶家族(NIMA relatedprotein kinases)基因(83%),331的核苷酸序列(80%)同源于与拟南芥(Ambidopsis thaliana)中编码内切-1,4-β-葡聚糖酶(EGases)的基因,313的核苷酸序列(78%)同源于与柑橘(Citrus sinensis)中的几丁质酶(chitinase CHI1)基因.提示这些基因片段在龙眼成花逆转过程中参与器官脱落、生殖生长和营养生长等的调控.
이용안(Dimocarpus longan Lour.CV.Longyou)정상성화화아화성화역전화아위재료,응용cDNA-AFLP기술.통과64대인물조합적cDNA-AFLP분석획득료2 560조확증편단,선택유명현차이적편단진행회수화측서,획득료14조핵감산서렬,기중유13개편단재GenBank수거고중발현동원서렬,1개공능미지.통과핵산화안기산비대분석,112기인편단동원우은회양(Populustremula × Populus alba)중편마Nek격매가족(NIMA relatedprotein kinases)기인(83%),331적핵감산서렬(80%)동원우여의남개(Ambidopsis thaliana)중편마내절-1,4-β-포취당매(EGases)적기인,313적핵감산서렬(78%)동원우여감귤(Citrus sinensis)중적궤정질매(chitinase CHI1)기인.제시저사기인편단재용안성화역전과정중삼여기관탈락、생식생장화영양생장등적조공.
In order to investigate the molecular mechanism of flowering reversion in Longan(Dimocarpus longan Lour.cv.Longyou),differential expression profile of cDNA between the longan normal flowering and flowering reversion was analyzed by cDNA-AFLP.Sixty-four pairs ofprimer were used for the eDNA-AFLP amplification and totally 2 560 differentially expressed fragments were detected.Fragments with obvious difference were selected to re-amplification and purification,sequenced,and 14 sequences were obtained.According to the alignment of Blastn and Biastx,differentially expressed 112 gene fragments were 83%identical in nucleotides to Populus tremula×Populus alba NIMA-related protein kinase mRNA and 331 were 80% identical in nucleotides to Arabidopsis thaliana xyloglucan endo-1,4-beta-D-glucanase precursor mRNA and 313 were 78%identical in nucleotides to Citrus sinensis chitinase CHI1(chil)mRNA,indicating that these gene fragments relate to organ fall off,procreaction growth and nutrition growth during the flowering reversion in longan.