CAJ | 학술논문

目的建立人血浆中抗病毒药物更昔洛韦的高效液相色谱快速分析方法.方法在一定pH值下用甲醇和二氯甲烷处理血浆,高速离心后外标法直接对上清液进行更昔洛韦定性、定量分析.以正常人血浆为空白样本添加标准更昔洛书,优化色谱分析条件并确定检材的前处理方法、工作曲线、线性范围、方法的精密度和同收率.以所建方法对12位受试者血浆中更昔洛韦代谢浓度进行12h监测.结果该方法分析血浆中的更昔洛韦,其线性范围为0.05 μg/mL～1.60 μg/mL;最低检测限为0.01 μg/mL(S/N≥3),日内、日间RSD≤5.1%,加样回收率在90.0%～95.4%之间.结论该分析方法操作便捷、实用、准确度高.适用于更昔洛韦的临床血药浓度监测和法医学药(毒)物快速鉴定.
목적 건립인혈장중항병독약물경석락위적고효액상색보쾌속분석방법.방법 재일정pH치하용갑순화이록갑완처리혈장,고속리심후외표법직접대상청액진행경석락위정성、정량분석.이정상인혈장위공백양본첨가표준경석낙서,우화색보분석조건병학정검재적전처리방법、공작곡선、선성범위、방법적정밀도화동수솔.이소건방법대12위수시자혈장중경석락위대사농도진행12h감측.결과 해방법분석혈장중적경석락위,기선성범위위0.05 μg/mL～1.60 μg/mL;최저검측한위0.01 μg/mL(S/N≥3),일내、일간RSD≤5.1%,가양회수솔재90.0%～95.4%지간.결론 해분석방법조작편첩、실용、준학도고.괄용우경석락위적림상혈약농도감측화법의학약(독)물쾌속감정.
Objective To develop a method for the determination of ganciclover in human plasma by RPHPLC.Methods Plasma containing ganciclover was extracted with methanol and methylene chloride,qualitative and quantitative analysis was carried out directly.Working curve,linear range,recovery,precision and so on was obtained according to the sample pre-processing method and analysis state.The HPLC method has been taken to investigate the plasma concentration of ganciclover for 12 volunteers.Results The relationship of the peak area of ganciclover concentration in plasma linear within the range of 0.05 μg/mL～1.60 μg/mL(r=0.9999).The lowest detection limit was 0.01 μg/mL(S/N≥13).The intra and inter-day RSD were less than 5.1%respectively.The recovery is about 90.0%～95.4%.Conclusion The established method in the article was shown to be sensitive,accurate and simple for the determination of ganciclover level.It is suitable for clinical detection of ganciclover and forensic medicine and toxicology analysis.