CAJ | 학술논문

利用EMS诱变育成优良籼型恢复系缙恢10号,从其后代中鉴定出一个白色条斑花叶突变体st(t),在三叶期开始表现白斑,拔节期白斑变为不规则线状,一直保持到成熟.突变体叶绿素含量明显下降,类胡萝卜素含量显著升高.透射电镜观察表明,突变体的绿色叶片部位与野生型相比,在细胞结构上无明显差异,叶绿体发育正常;突变体的白化部位细胞结构异常,质体内多含有积聚在一起的嗜锇小球,不能发育出正常叶绿体所具有的类囊体和基质片层结构.遗传分析表明该性状受一对隐性核基因调控,利用1 500株西农1A/st(t)的F_2隐性定位群体,最终把St(t)基因定位在第6染色体SSR标记RM19745和RM19762之间,遗传距离分别为0.07 cM和0.27 cM,根据9311基因组序列推测,两标记之间的物理距离约为345 kb.这为St(t)基因的图位克隆和分子标记辅助育种奠定了基础.
이용EMS유변육성우량선형회복계진회10호,종기후대중감정출일개백색조반화협돌변체st(t),재삼협기개시표현백반,발절기백반변위불규칙선상,일직보지도성숙.돌변체협록소함량명현하강,류호라복소함량현저승고.투사전경관찰표명,돌변체적록색협편부위여야생형상비,재세포결구상무명현차이,협록체발육정상;돌변체적백화부위세포결구이상,질체내다함유적취재일기적기철소구,불능발육출정상협록체소구유적류낭체화기질편층결구.유전분석표명해성상수일대은성핵기인조공,이용1 500주서농1A/st(t)적F_2은성정위군체,최종파St(t)기인정위재제6염색체SSR표기RM19745화RM19762지간,유전거리분별위0.07 cM화0.27 cM,근거9311기인조서렬추측,량표기지간적물리거리약위345 kb.저위St(t)기인적도위극륭화분자표기보조육충전정료기출.
A stripe leaf mutant temporarily designated as st(t) was discovered in the progeny of an excellent indica restorer line Jinhui10 seed by treatment of EMS (ethyl methane sulfonate) in Rice Research Institute of Southwest University. The st(t) mutant displayed the variegated leaves at three-leaf stage, the variegation developed the white irregular line stripe from elongating stage to the maturation. Compared with the wild type, the mutant decreased the chlorophyll content and increased the carotenoid content significantly. Cells structure was detected by Transmission Electron Microscopic (TEM), and the results indicated that there was no difference between the wild type and the green parts of the st(t), both of them could develop normal chloroplast; in the white parts of the st(t), cells developed abnormally contained irregular stromal lamellae, aberrant thylakoids and more osmiophilic gran-ules. Genetic analysis suggested that the mutational characters were controlled by one nuclear recessive gene. Xinong 1A was crossed with the st(t) and 1 500 mutants from the F_2 population were used for gene mapping. Finally, St(t) gene was mapped be-tween SSR marker RM19745 and RM19762 on the chromosome 6, the genetic distances were 0.07 cM and 0.27 cM respectively and the physical distance was 345 kb according to indica rice 9311. This result provides a foundation of map-based cloning of St(t) gene as well as its molecular marker-assisted breeding.