CAJ | 학술논문

目的:探讨肝癌细胞中间隙连接蛋白Connexin32对肝癌细胞运动和侵袭能力的影响.方法:利用逆病毒感染的方法在肝癌细胞系Li-7中建立Connexin32蛋白表达可通过doxycycline调控的Li-7 Tet-off Cx32稳定克隆,运用体外transwell migra-tion and invasion assay方法验证Connexin32表达与肝癌细胞运动和侵袭能力的相关性.结果:建立稳定整合了Connexin32 cDNA 和调控序列Tet-off的肝癌细胞Li-7亚克隆,Western-blot结果显示外源性Connexin32蛋白表达于细胞浆中.亚细胞定位分析证实Connexin32蛋白定位于高尔基复合体内,此细胞浆内Connexin32蛋白过表达显著增强肝癌细胞的体外运动和侵袭能力.结论:肝癌细胞浆内异位表达的Connexin32蛋白发挥促进肿瘤进展的作用.
목적:탐토간암세포중간극련접단백Connexin32대간암세포운동화침습능력적영향.방법:이용역병독감염적방법재간암세포계Li-7중건립Connexin32단백표체가통과doxycycline조공적Li-7 Tet-off Cx32은정극륭,운용체외transwell migra-tion and invasion assay방법험증Connexin32표체여간암세포운동화침습능력적상관성.결과:건립은정정합료Connexin32 cDNA 화조공서렬Tet-off적간암세포Li-7아극륭,Western-blot결과현시외원성Connexin32단백표체우세포장중.아세포정위분석증실Connexin32단백정위우고이기복합체내,차세포장내Connexin32단백과표체현저증강간암세포적체외운동화침습능력.결론:간암세포장내이위표체적Connexin32단백발휘촉진종류진전적작용.
Objective:To explorer the effects of gap junction protein connexin32 on motility and invasiveness of hepato-cellular carcinoma cells.Methods:The stable subclone Li-7 Tet-off Cx32 in which connexin32 expression could be controlled by doxycyclin was established in hepatocellualr carcinoma Li-7 cells by retrovirus infection.Cell motility and invasive ability was analyzed by in vitro transwell motility and invasion assay.Results:A subclone originated from Li-7 cells was established,in which both connexin32 cDNA and regulatory element Tet-off were stably integrated.Western-blot results showed that exogeneous connexin32 was expressed in the cytoplasm,mainly in Golgi apparatus,and the abnormal accumulation of connexin32 protein in the cytoplasm could significantly promote motility and invasiveness of Li-7 cells.Conclusion:Cytoplasmic accumulation of connexin32 protein can promote tumor progression in Li-7 hepatocellular carcinoma cells.