CAJ | 학술논문

目的研究毕赤酵母表达的重组HBsAg(Pichia-rHBsAg)在小鼠中诱导T细胞免疫应答的能力,评价疫苗的免疫原性.方法以rHBsAg免疫雌性BALB/c小鼠,制备小鼠脾淋巴细胞,并在体外以抗原/特异性多肽刺激.采用ELISA法测定抗原特异性T淋巴细胞分泌的细胞因子,酶联免疫斑点法(ELISPOT)测定CTL频数.结果由毕赤酵母表达的rHBsAg可在小鼠中诱导Th1和Th2型免疫应答;加铝佐剂的rHBsAg诱导IFN-γ的水平及CTL克隆明显高于无佐剂抗原;Pichia-rHBsAg刺激小鼠细胞免疫应答的水平强于酵母-rHBsAg,与中国仓鼠卵巢细胞-rHBsAg相仿.结论 Pichia-rHBsAg可在BALB/c小鼠中诱导较强的细胞免疫应答.
목적 연구필적효모표체적중조HBsAg(Pichia-rHBsAg)재소서중유도T세포면역응답적능력,평개역묘적면역원성.방법 이rHBsAg면역자성BALB/c소서,제비소서비림파세포,병재체외이항원/특이성다태자격.채용ELISA법측정항원특이성T림파세포분비적세포인자,매련면역반점법(ELISPOT)측정CTL빈수.결과 유필적효모표체적rHBsAg가재소서중유도Th1화Th2형면역응답;가려좌제적rHBsAg유도IFN-γ적수평급CTL극륭명현고우무좌제항원;Pichia-rHBsAg자격소서세포면역응답적수평강우효모-rHBsAg,여중국창서란소세포-rHBsAg상방.결론 Pichia-rHBsAg가재BALB/c소서중유도교강적세포면역응답.
Objective To study the response of T cell-mediated immunity (CMI) induced with HBsAg expressed in Pichia pastoris (Pichia-rHBsAg) in mice and evaluate the immunogenicity of a vaccine. Methods BALB/c mice were immunized with rHBsAg with or without an adj uvant.Then spleenlymphocytes were isolated and stimulated by antigen/specific peptide for investigating CMI response.Cytokines and CTL frequency were detected by ELISA and ELISPOT.respectively. Results PichiarHBsAg could trigger Th1 and Th2 type immune response.Comparing with rHBsAg alone,higher concentration of IFN-γ and more CTL clones were observed with rHBsAg plus AI(0H)3 aajuvant.In addition,the CMI response elicited by Pichia-rHBsAg is stronger than that induced by yeast-rHBsAg,and comparable to that induced by CHO-rHBsAg. Conclusion Pichia-rHBsAg could induce stronger CMI response in BALB/c mice.