中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2008年
4期
627-628
,共2页
血液透析滤过%T淋巴细胞亚群%红细胞生成素
血液透析濾過%T淋巴細胞亞群%紅細胞生成素
혈액투석려과%T림파세포아군%홍세포생성소
Hemodia filtration%T-lymphocyte subsets%Erythropoietin
目的 通过对红细胞生成素(rHuEPO)不同反应的患者用流式细胞仪检测T细胞亚群以了解rHuEPO低反应与细胞免疫的关系及可能的作用机制.方法 将40例维持性血液透析(MHD)患者按照rHuEPO反应的不同分为第一组和第二组,rHuEPO反应低下的18例为第一组,rHuEPO反应良好的22例为第二组,另设一健康对照组20例.应用流式细胞仪检测各组患者T淋巴细胞表面CD+4、CD+8、CD+4/CD+28、CD+8/CD+28抗原的表达情况.结果 rHuEPO反应良好组和反应低下组的CD+4 T细胞、CD+8 T细胞百分比差异无统计学意义,但均显著低于对照组;rHuEPO反应低下组的CD+4 CD+28/CD+4、CD+8 CD+28/CD+8 T细胞的百分比明显低于反应良好组和对照组,而反应良好组与对照组之间无明显差异.结论 尿毒症MHD患者存在T细胞免疫表型的改变.
目的 通過對紅細胞生成素(rHuEPO)不同反應的患者用流式細胞儀檢測T細胞亞群以瞭解rHuEPO低反應與細胞免疫的關繫及可能的作用機製.方法 將40例維持性血液透析(MHD)患者按照rHuEPO反應的不同分為第一組和第二組,rHuEPO反應低下的18例為第一組,rHuEPO反應良好的22例為第二組,另設一健康對照組20例.應用流式細胞儀檢測各組患者T淋巴細胞錶麵CD+4、CD+8、CD+4/CD+28、CD+8/CD+28抗原的錶達情況.結果 rHuEPO反應良好組和反應低下組的CD+4 T細胞、CD+8 T細胞百分比差異無統計學意義,但均顯著低于對照組;rHuEPO反應低下組的CD+4 CD+28/CD+4、CD+8 CD+28/CD+8 T細胞的百分比明顯低于反應良好組和對照組,而反應良好組與對照組之間無明顯差異.結論 尿毒癥MHD患者存在T細胞免疫錶型的改變.
목적 통과대홍세포생성소(rHuEPO)불동반응적환자용류식세포의검측T세포아군이료해rHuEPO저반응여세포면역적관계급가능적작용궤제.방법 장40례유지성혈액투석(MHD)환자안조rHuEPO반응적불동분위제일조화제이조,rHuEPO반응저하적18례위제일조,rHuEPO반응량호적22례위제이조,령설일건강대조조20례.응용류식세포의검측각조환자T림파세포표면CD+4、CD+8、CD+4/CD+28、CD+8/CD+28항원적표체정황.결과 rHuEPO반응량호조화반응저하조적CD+4 T세포、CD+8 T세포백분비차이무통계학의의,단균현저저우대조조;rHuEPO반응저하조적CD+4 CD+28/CD+4、CD+8 CD+28/CD+8 T세포적백분비명현저우반응량호조화대조조,이반응량호조여대조조지간무명현차이.결론 뇨독증MHD환자존재T세포면역표형적개변.
Objective One hypothesis is that patients with uraemia showing hyporesponsiveness to rHuEPO may have enhanced levels of immune activation causing increased rdease of inflammatory cytokines. To study the effects of chronic inflammation on the hyporesponsiveness to rHuEPO and its possible mechanisms, T cell phenotypes using flow cytometry and the relationship between these changes were detected. Methods 40 patients with MHD and 20 age-matched healthy volunteers were enrolled in the study. The MHD patients were divided into two groups according to the responsiveness to rHuEPO:22 cases with good responders and 18 cases with poor responders. The percentages of T cell subsets including CD+4 ,CD+8,CD+4/CD+28,CD+8/CD+28 T cell using flow cytometry were detected. Results The percentages of CD+4 and CD+8 T cell were lower in MHD patients than that in control group. The percentages of CD4+/CD+28 and CD+8/CD+28 T cell were lower in group of poor responders than in good responders.Conclusion Chronic inflammation state is common in MIlD patients. The percentages of CD+4/CD+28 and CD+8/CD+28 T cell in poor responders were much lower than good responders. The changes of T cell phenotypes may play a role in pathogenesis of hyporespousiveness to rHuEPO.
