中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
1期
67-70
,共4页
丁力%严国章%石翊飒%马永丰
丁力%嚴國章%石翊颯%馬永豐
정력%엄국장%석익삽%마영봉
外科手术%创伤和损伤%老年人%认知障碍%海马%突触
外科手術%創傷和損傷%老年人%認知障礙%海馬%突觸
외과수술%창상화손상%노년인%인지장애%해마%돌촉
Surgical procedures,operative%Wounds and injuries%Aged%Cognition disorders%Hippocampus%Synapses
目的 探讨手术创伤对老龄大鼠海马CA3区突触结构的影响.方法 健康SD大鼠56只,月龄18月,随机分为3组,对照组(C组,n=8)腹腔注射生理盐水0.8 ml/kg;麻醉组(A组,n=24)腹腔注射氯胺酮40 mg/kg;手术组(O组,n=24)腹腔注射氯胺酮40 mg/kg,翻正反射消失后行脾脏切除术.A组和O组于麻醉或术后1、3,7 d(T_(1～3))时取8只大鼠行Morris水迷宫实验测试认知功能,并测定海马CA3区突触结构各指标.结果 与C组和A组比较,O组T_(1,2)时通过原平台次数、突触数减少,突触间隙增宽,突触后膜致密物厚度变薄,突触活性带长度缩短,突触界面曲率减小(P<0.05或0.01).与C组比较,A组T_1时、O组T_(1,2)时潜伏期及游泳距离延长(P<0.01);与A组比较,O组T_(1,2)时潜伏期延长,T_2时游泳距离延长(P<0.05).结论 手术创伤导致老龄大鼠术后早期认知功能减退的机制与海马CA3区突触结构改变有关.
目的 探討手術創傷對老齡大鼠海馬CA3區突觸結構的影響.方法 健康SD大鼠56隻,月齡18月,隨機分為3組,對照組(C組,n=8)腹腔註射生理鹽水0.8 ml/kg;痳醉組(A組,n=24)腹腔註射氯胺酮40 mg/kg;手術組(O組,n=24)腹腔註射氯胺酮40 mg/kg,翻正反射消失後行脾髒切除術.A組和O組于痳醉或術後1、3,7 d(T_(1～3))時取8隻大鼠行Morris水迷宮實驗測試認知功能,併測定海馬CA3區突觸結構各指標.結果 與C組和A組比較,O組T_(1,2)時通過原平檯次數、突觸數減少,突觸間隙增寬,突觸後膜緻密物厚度變薄,突觸活性帶長度縮短,突觸界麵麯率減小(P<0.05或0.01).與C組比較,A組T_1時、O組T_(1,2)時潛伏期及遊泳距離延長(P<0.01);與A組比較,O組T_(1,2)時潛伏期延長,T_2時遊泳距離延長(P<0.05).結論 手術創傷導緻老齡大鼠術後早期認知功能減退的機製與海馬CA3區突觸結構改變有關.
목적 탐토수술창상대노령대서해마CA3구돌촉결구적영향.방법 건강SD대서56지,월령18월,수궤분위3조,대조조(C조,n=8)복강주사생리염수0.8 ml/kg;마취조(A조,n=24)복강주사록알동40 mg/kg;수술조(O조,n=24)복강주사록알동40 mg/kg,번정반사소실후행비장절제술.A조화O조우마취혹술후1、3,7 d(T_(1～3))시취8지대서행Morris수미궁실험측시인지공능,병측정해마CA3구돌촉결구각지표.결과 여C조화A조비교,O조T_(1,2)시통과원평태차수、돌촉수감소,돌촉간극증관,돌촉후막치밀물후도변박,돌촉활성대장도축단,돌촉계면곡솔감소(P<0.05혹0.01).여C조비교,A조T_1시、O조T_(1,2)시잠복기급유영거리연장(P<0.01);여A조비교,O조T_(1,2)시잠복기연장,T_2시유영거리연장(P<0.05).결론 수술창상도치노령대서술후조기인지공능감퇴적궤제여해마CA3구돌촉결구개변유관.
Objective To investigate the effects of surgical trauma on synaptic structure in hippocampal CA3 area in aged rats.Methods Fifty-six healthy SD rats aged 18 months were randomly divided into 3 groups: control group (group C, n = 8) , anesthesia group (group A, n = 24) , and operation group (group O, n - 24) . Anesthesia was performed with intraperitoneal ketamine 40 mg/kg but no operation was carried out in group A. Anesthesia was also performed with intraperitoneal ketamine 40 mg/kg, and splenectomy was performed after loss of righting reflex in group O. Eight animals from group A and O selected on 1, 3, and 7 d after anesthesia or operation respectively underwent Morris water maze test for assessment of the cognitive function. The animals were . then decapitated. Hippocampal CA3 area was isolated for examination with electron microscope and the synaptic structure in the polymorphic layer of hippocampal CA3 area was measured. Results Compared to group C and A, the times of passing through the original platform and number of synapses were significantly reduced, the width of synaptic cleft was significantly increased, the thickness of the postsynaptic density was significantly decreased, the length of the active zones was significantly shortened, and the curvature of the synaptic interface and percentage of perforated synapses were significantly decreased at T_(1,2), ( P < 0.05 or 0.01) , but no significant change was found in the indices mentioned above at T_3 in group O(P > 0.05). Compared to group C, the latency and swimming distance were significantly prolonged at T_1 in group A and at T_(1,2) in group O ( P < 0.01) . Compared to group A, the latency at T_(1,2) and the swimming distance at T_2 were significantly prolonged in group O ( P < 0.05) . Conclusion Surgical trauma can induce early postoperative cognitive impairment through changing synaptic structure in hippocampal CA3 area in aged rats.
