CAJ | 학술논문

目的讨Ⅰ型干扰素(IFN)诱导基因(IFIG)的表达与系统性红斑狼疮(SLE)的临床相关性.方法实时定量聚合酶链反应(PCR)检测67例SLE患者及23名健康对照外周血白细胞中5个IFIG(OAS-1、Mx-1、Lv6e、IFIT1和IFIT4)的mRNA水平,计算IFN积分,并与相应临床资料进行统计学分析;随机挑取17例患者采用荧光素酶报告基因测定其血清IFN活性并与IFN积分行相关分析.结果 IFN积分在SLE患者中显著增高,并与血清IFN活性及SLE疾病活动指数(SLEDAI)-2K积分呈显著正相关,与补体C3水平呈负相关.此外,抗Sm、抗RNP抗体阳性的患者中存在IFN积分显著升高.结论 IFN积分与血清IFN活性密切相关,与SLE的疾病活动性及特定自身抗体表型相关,是反映SLE活动的新的生物标志物.
목적 토Ⅰ형간우소(IFN)유도기인(IFIG)적표체여계통성홍반랑창(SLE)적림상상관성.방법 실시정량취합매련반응(PCR)검측67례SLE환자급23명건강대조외주혈백세포중5개IFIG(OAS-1、Mx-1、Lv6e、IFIT1화IFIT4)적mRNA수평,계산IFN적분,병여상응림상자료진행통계학분석;수궤도취17례환자채용형광소매보고기인측정기혈청IFN활성병여IFN적분행상관분석.결과 IFN적분재SLE환자중현저증고,병여혈청IFN활성급SLE질병활동지수(SLEDAI)-2K적분정현저정상관,여보체C3수평정부상관.차외,항Sm、항RNP항체양성적환자중존재IFN적분현저승고.결론 IFN적분여혈청IFN활성밀절상관,여SLE적질병활동성급특정자신항체표형상관,시반영SLE활동적신적생물표지물.
Objective To correlate the expression levels of interferon inducible genes (IFIGs) with disease activity and clinical features in systemic lupus erythematosus (SLE) patiems,.Methods Peripheral blood cells obtained from 67 SLE patients and 23 healthy donors (HDs) were subjected to real-time PCR to measure the transcriptional levels of five IFIGs (OAS-1,Mx-1,Ly6e,IFIT1 and IFIT4).Interferon scores were calculated and were compared between various groups of SLE patients as well as between patients and controls;ISRE lucife:rase reporter gene activity was measured in 17 of 67 patients and correlated with interferon score.Results Interferon scores were strongly correlated with ISRIE reporter gene aetivity,which represented for the type Ⅰ interferon activity in serum.The expression.levels of IFIGs and jinterferon scores were significantly elevated in SLE patients compared with HDs (P＜0.0001).Interferon scores were correlated positively with SLEDAI-2K(P=0.0006) and negatively with C3 levels(P=0.0162).Interferon scores were also significantly elevated in SLE patients with a positive anti-Sm or anti-RNP autoantibodies.Clonclusion The interferon score may be regarded as a good indicator for serum type I interferon activity in SLE and serves as a new hiomarker for disease activity in SLE patients.