中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2009年
2期
84-88
,共5页
陈小青%扶琼%顾越英%叶霜%崔慧娟%郭彦芝
陳小青%扶瓊%顧越英%葉霜%崔慧娟%郭彥芝
진소청%부경%고월영%협상%최혜연%곽언지
红斑狼疮,系统性%Ⅰ型干扰素%生物标志物
紅斑狼瘡,繫統性%Ⅰ型榦擾素%生物標誌物
홍반랑창,계통성%Ⅰ형간우소%생물표지물
Lupus erythematosus,systemic%Type I interferon%Biomarker
目的 讨Ⅰ型干扰素(IFN)诱导基因(IFIG)的表达与系统性红斑狼疮(SLE)的临床相关性.方法 实时定量聚合酶链反应(PCR)检测67例SLE患者及23名健康对照外周血白细胞中5个IFIG(OAS-1、Mx-1、Lv6e、IFIT1和IFIT4)的mRNA水平,计算IFN积分,并与相应临床资料进行统计学分析;随机挑取17例患者采用荧光素酶报告基因测定其血清IFN活性并与IFN积分行相关分析.结果 IFN积分在SLE患者中显著增高,并与血清IFN活性及SLE疾病活动指数(SLEDAI)-2K积分呈显著正相关,与补体C3水平呈负相关.此外,抗Sm、抗RNP抗体阳性的患者中存在IFN积分显著升高.结论 IFN积分与血清IFN活性密切相关,与SLE的疾病活动性及特定自身抗体表型相关,是反映SLE活动的新的生物标志物.
目的 討Ⅰ型榦擾素(IFN)誘導基因(IFIG)的錶達與繫統性紅斑狼瘡(SLE)的臨床相關性.方法 實時定量聚閤酶鏈反應(PCR)檢測67例SLE患者及23名健康對照外週血白細胞中5箇IFIG(OAS-1、Mx-1、Lv6e、IFIT1和IFIT4)的mRNA水平,計算IFN積分,併與相應臨床資料進行統計學分析;隨機挑取17例患者採用熒光素酶報告基因測定其血清IFN活性併與IFN積分行相關分析.結果 IFN積分在SLE患者中顯著增高,併與血清IFN活性及SLE疾病活動指數(SLEDAI)-2K積分呈顯著正相關,與補體C3水平呈負相關.此外,抗Sm、抗RNP抗體暘性的患者中存在IFN積分顯著升高.結論 IFN積分與血清IFN活性密切相關,與SLE的疾病活動性及特定自身抗體錶型相關,是反映SLE活動的新的生物標誌物.
목적 토Ⅰ형간우소(IFN)유도기인(IFIG)적표체여계통성홍반랑창(SLE)적림상상관성.방법 실시정량취합매련반응(PCR)검측67례SLE환자급23명건강대조외주혈백세포중5개IFIG(OAS-1、Mx-1、Lv6e、IFIT1화IFIT4)적mRNA수평,계산IFN적분,병여상응림상자료진행통계학분석;수궤도취17례환자채용형광소매보고기인측정기혈청IFN활성병여IFN적분행상관분석.결과 IFN적분재SLE환자중현저증고,병여혈청IFN활성급SLE질병활동지수(SLEDAI)-2K적분정현저정상관,여보체C3수평정부상관.차외,항Sm、항RNP항체양성적환자중존재IFN적분현저승고.결론 IFN적분여혈청IFN활성밀절상관,여SLE적질병활동성급특정자신항체표형상관,시반영SLE활동적신적생물표지물.
Objective To correlate the expression levels of interferon inducible genes (IFIGs) with disease activity and clinical features in systemic lupus erythematosus (SLE) patiems,.Methods Peripheral blood cells obtained from 67 SLE patients and 23 healthy donors (HDs) were subjected to real-time PCR to measure the transcriptional levels of five IFIGs (OAS-1,Mx-1,Ly6e,IFIT1 and IFIT4).Interferon scores were calculated and were compared between various groups of SLE patients as well as between patients and controls;ISRE lucife:rase reporter gene activity was measured in 17 of 67 patients and correlated with interferon score.Results Interferon scores were strongly correlated with ISRIE reporter gene aetivity,which represented for the type Ⅰ interferon activity in serum.The expression.levels of IFIGs and jinterferon scores were significantly elevated in SLE patients compared with HDs (P<0.0001).Interferon scores were correlated positively with SLEDAI-2K(P=0.0006) and negatively with C3 levels(P=0.0162).Interferon scores were also significantly elevated in SLE patients with a positive anti-Sm or anti-RNP autoantibodies.Clonclusion The interferon score may be regarded as a good indicator for serum type I interferon activity in SLE and serves as a new hiomarker for disease activity in SLE patients.