中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2010年
3期
151-153,后插1
,共4页
王庆文%曾惠芬%刘郁%杨彩红%曾沛英%陈澄%尹为华%余光银%蔡广玲%蓝辉耀
王慶文%曾惠芬%劉鬱%楊綵紅%曾沛英%陳澄%尹為華%餘光銀%蔡廣玲%藍輝耀
왕경문%증혜분%류욱%양채홍%증패영%진징%윤위화%여광은%채엄령%람휘요
脊柱炎,强直性%转化生长因子β%骶髂关节
脊柱炎,彊直性%轉化生長因子β%骶髂關節
척주염,강직성%전화생장인자β%저가관절
Spondylitis,ankylosing%Transforming growth factor beta%Sacroiliac joint
目的 了解强直性脊柱炎(AS)骶髂关节中转化生长因子(TGF)-β_1/Smad信号转导通路中主要分子的表达情况,探讨TGF-β_1/Smad信号转导在AS发病机制中的作用.方法 53例AS患者均检测血清TGF-β_1.及红细胞沉降率(ESR)、C反应蛋白(CRP)水平.其中30例行CT导引下穿刺活检取得骶髂关节组织,通过免疫组织化学方法,检测TGF-β_1、p-Smad3、Smad7的表达.采用多个样本均数的单因素方差分析、两样本均数的t检验以及Kolmogorov-Smimov检验进行统计学处理.结果 AS患者中ESR/CRP升高组血清TGF-β_1(15.9±5.6)ng/ml,较健康对照组、ESR/CRP健康组[(5.4±5.8)ng/ml和(4.1±3.6)ng/ml]均明显升高.与健康对照组相比,AS骶髂关节组织可见TGF-β_1高表达,主要在血管翳中炎症细胞的胞质中表达;Smad7明显低表达;p-smad3则主要表达于骨髓及血管翳中炎症细胞的细胞核中,提示smad3已被激活.结论 AS中存在TGF-β_1的过高表达,Smad信号通路的激活,Samd7的低表达,可能与AS骶髂关节炎症活动、软骨的纤维化变性有关.
目的 瞭解彊直性脊柱炎(AS)骶髂關節中轉化生長因子(TGF)-β_1/Smad信號轉導通路中主要分子的錶達情況,探討TGF-β_1/Smad信號轉導在AS髮病機製中的作用.方法 53例AS患者均檢測血清TGF-β_1.及紅細胞沉降率(ESR)、C反應蛋白(CRP)水平.其中30例行CT導引下穿刺活檢取得骶髂關節組織,通過免疫組織化學方法,檢測TGF-β_1、p-Smad3、Smad7的錶達.採用多箇樣本均數的單因素方差分析、兩樣本均數的t檢驗以及Kolmogorov-Smimov檢驗進行統計學處理.結果 AS患者中ESR/CRP升高組血清TGF-β_1(15.9±5.6)ng/ml,較健康對照組、ESR/CRP健康組[(5.4±5.8)ng/ml和(4.1±3.6)ng/ml]均明顯升高.與健康對照組相比,AS骶髂關節組織可見TGF-β_1高錶達,主要在血管翳中炎癥細胞的胞質中錶達;Smad7明顯低錶達;p-smad3則主要錶達于骨髓及血管翳中炎癥細胞的細胞覈中,提示smad3已被激活.結論 AS中存在TGF-β_1的過高錶達,Smad信號通路的激活,Samd7的低錶達,可能與AS骶髂關節炎癥活動、軟骨的纖維化變性有關.
목적 료해강직성척주염(AS)저가관절중전화생장인자(TGF)-β_1/Smad신호전도통로중주요분자적표체정황,탐토TGF-β_1/Smad신호전도재AS발병궤제중적작용.방법 53례AS환자균검측혈청TGF-β_1.급홍세포침강솔(ESR)、C반응단백(CRP)수평.기중30례행CT도인하천자활검취득저가관절조직,통과면역조직화학방법,검측TGF-β_1、p-Smad3、Smad7적표체.채용다개양본균수적단인소방차분석、량양본균수적t검험이급Kolmogorov-Smimov검험진행통계학처리.결과 AS환자중ESR/CRP승고조혈청TGF-β_1(15.9±5.6)ng/ml,교건강대조조、ESR/CRP건강조[(5.4±5.8)ng/ml화(4.1±3.6)ng/ml]균명현승고.여건강대조조상비,AS저가관절조직가견TGF-β_1고표체,주요재혈관예중염증세포적포질중표체;Smad7명현저표체;p-smad3칙주요표체우골수급혈관예중염증세포적세포핵중,제시smad3이피격활.결론 AS중존재TGF-β_1적과고표체,Smad신호통로적격활,Samd7적저표체,가능여AS저가관절염증활동、연골적섬유화변성유관.
Objective To investigate the expression of transforming growth factor beta (TGF-β) and Smad signaling in ankylosing spondylitis (AS) and to explore their roles in the pathogenesis. Methods Fiftythree patients with AS were included in the study. In these 53 cases, 30 patients were performed computed tomography-guided needle biopsy in sacroiliac joint. Serum TGF-β_1 was determined by enzyme-linked immunosorbent assay (ELISA). Immunohistological studies were performed with the streptavidin-peroxidase conjugated methods to assess the expression of TGF-β_1, p-smad3 and Smad7 in sacroiliac joint tissue sample.One-way ANOVA, two independent samples t test and kolmogoorov-Simimov test were used to do statistical analysis. Results In 53 cases patients with AS, 20 cases were with high level Erythro-cyte sedimentation rate(ESR) and C-reactive protein (CRP), while those of the other 33 cases were normal. Serum average TGF-β_1level [ (15.9±5.6) ng/ml ], in patients with high level ESR/CRP [(5.4±5.8) ng/ml ] was significantly increased as compared to the controls and patients with normal ESR/CRP [(4.1±3.6) ng/ml] (P<0.05). There was no expression of TGF-β_1 could be detected in the pannus and bone marrow in SI joints tissue of 30 cases with AS, while decreased level of smad7 expression was detected. In addition, p-smad3 expression was found in the nuclear. Conclusion TGF-β_1 signaling may play an important role in the inflammatory erosion and cartilage fibrosis of sacrojlitis in AS.