CAJ | 학술논문

目的研究反式作用因子与心力衰竭相关蛋白表达量变化的关系。方法运用生物信息学方法对4种心力衰竭相关蛋白质的基因序列5＇调控区进行反式作用因子定位分析，应用免疫组化法检测40例尸检心肌标本中血清反应因子水平。结果通过定位分析，预测出9种反式作用因子可能在心力衰竭发生发展过程中对心力衰竭相关蛋白发挥转录调控作用，选择其中的血清反应因子(SRF)为例，经免疫组化分析得出：正常成人心脏中SRF量处于高水平，SRF阳性细胞百分率为(91.6±10.05)％；在慢性心力衰竭患者中SRF表达水平明显降低，仅(28.73±12.79)％，且SRF水平与心功能分级呈负相关；在急性心肌梗死患者中，SRF表达水平较慢性心力衰竭者高，为(45.33±13.30)％，较正常组仍明显降低。结论血清反应因子在心力衰竭发生发展过程中通过其自身量的变化对心力衰竭相关蛋白表达起转录调控作用。
목적연구반식작용인자여심력쇠갈상관단백표체량변화적관계。방법운용생물신식학방법대4충심력쇠갈상관단백질적기인서렬5＇조공구진행반식작용인자정위분석，응용면역조화법검측40례시검심기표본중혈청반응인자수평。결과통과정위분석，예측출9충반식작용인자가능재심력쇠갈발생발전과정중대심력쇠갈상관단백발휘전록조공작용，선택기중적혈청반응인자(SRF)위례，경면역조화분석득출：정상성인심장중SRF량처우고수평，SRF양성세포백분솔위(91.6±10.05)％；재만성심력쇠갈환자중SRF표체수평명현강저，부(28.73±12.79)％，차SRF수평여심공능분급정부상관；재급성심기경사환자중，SRF표체수평교만성심력쇠갈자고，위(45.33±13.30)％，교정상조잉명현강저。결론혈청반응인자재심력쇠갈발생발전과정중통과기자신량적변화대심력쇠갈상관단백표체기전록조공작용。
Objective To investigate the relation ship between trans-acting factor and the change of protein expression levels in heart failure. Methods Bioinformationak methods were used to analyze the bindingsites in the 5＇ regulation regions of four genes of the protein expression levels change in heart failure. The serum response factor (SRF) level was determined by innuno-hischemical method. Results Nine trans-transcription factors were inferred to influence these protein expression levels in occurrence and development of heart failure. SRF was selected from the nine factors and assayed. The results showed a higher level of SRF in healthy people, the percentage of anti-SRF positive cardiocytes was(91.6±10.05)%. A lower percentage of SRF in congestive heart failure (CHF) was (28.73±12.79)%. And the level of SRF was associated with the functional class of the New York Heart Association. A relative higher level of SRF was found in the acute myocardial infarction (AMI) (4533±13.30)% than that in CHF and in the healthy. Conclusion It was suggested that the level of SRF plays a regulative role in CHF.