中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2010年
12期
1141-1143
,共3页
王宏勤%刘晓东%范益民%聂晓冬%白东%郝解贺
王宏勤%劉曉東%範益民%聶曉鼕%白東%郝解賀
왕굉근%류효동%범익민%섭효동%백동%학해하
神经胶质瘤%凋亡%PUMA%Bcl-2%Bax%蛋白免疫印迹法
神經膠質瘤%凋亡%PUMA%Bcl-2%Bax%蛋白免疫印跡法
신경효질류%조망%PUMA%Bcl-2%Bax%단백면역인적법
Glioma%Apoptosis%PUMA%Bcl-2%Bax%Western-blot
目的 探讨促凋亡基因PUMA在脑胶质瘤中的表达,及其与Bcl-2、Bax蛋白表达的相关性.方法 应用免疫印迹法(Western blot)检测10例正常脑组织及52例脑胶质瘤组织中PUMA、Bcl-2、Bax蛋白的表达,通过计算吸光度比值求得各蛋白相对表达量并统计分析结果.结果 (1)PUMA蛋白、Bax、Bcl-2蛋白表达在Ⅱ、Ⅲ、Ⅳ级胶质瘤中与正常脑组织差异有统计学意义(P＜0.01),Ⅰ级胶质瘤与对照组表达差异无统计学意义(P＞0.05).(2)病理分级与PUMA、Bax蛋白和Bcl-2蛋白表达相关(rPUMA=-0.925,rBax=-0.931,rBcl-2=0.945,P＜0.001).(3)PUMA和Bax、Bcl-2蛋白的表达相关(r1=0.963,r2=-0.937 P＜0.001).结论 PUMA可能是胶质瘤的一个重要的预后标记物.PUMA可通过促进肿瘤细胞凋亡,对脑胶质瘤的发生、发展起作用;PUMA可能与凋亡相关基因Bax、Bcl-2在脑胶质瘤的凋亡中分别起协同和拮抗作用.
目的 探討促凋亡基因PUMA在腦膠質瘤中的錶達,及其與Bcl-2、Bax蛋白錶達的相關性.方法 應用免疫印跡法(Western blot)檢測10例正常腦組織及52例腦膠質瘤組織中PUMA、Bcl-2、Bax蛋白的錶達,通過計算吸光度比值求得各蛋白相對錶達量併統計分析結果.結果 (1)PUMA蛋白、Bax、Bcl-2蛋白錶達在Ⅱ、Ⅲ、Ⅳ級膠質瘤中與正常腦組織差異有統計學意義(P＜0.01),Ⅰ級膠質瘤與對照組錶達差異無統計學意義(P＞0.05).(2)病理分級與PUMA、Bax蛋白和Bcl-2蛋白錶達相關(rPUMA=-0.925,rBax=-0.931,rBcl-2=0.945,P＜0.001).(3)PUMA和Bax、Bcl-2蛋白的錶達相關(r1=0.963,r2=-0.937 P＜0.001).結論 PUMA可能是膠質瘤的一箇重要的預後標記物.PUMA可通過促進腫瘤細胞凋亡,對腦膠質瘤的髮生、髮展起作用;PUMA可能與凋亡相關基因Bax、Bcl-2在腦膠質瘤的凋亡中分彆起協同和拮抗作用.
목적 탐토촉조망기인PUMA재뇌효질류중적표체,급기여Bcl-2、Bax단백표체적상관성.방법 응용면역인적법(Western blot)검측10례정상뇌조직급52례뇌효질류조직중PUMA、Bcl-2、Bax단백적표체,통과계산흡광도비치구득각단백상대표체량병통계분석결과.결과 (1)PUMA단백、Bax、Bcl-2단백표체재Ⅱ、Ⅲ、Ⅳ급효질류중여정상뇌조직차이유통계학의의(P＜0.01),Ⅰ급효질류여대조조표체차이무통계학의의(P＞0.05).(2)병리분급여PUMA、Bax단백화Bcl-2단백표체상관(rPUMA=-0.925,rBax=-0.931,rBcl-2=0.945,P＜0.001).(3)PUMA화Bax、Bcl-2단백적표체상관(r1=0.963,r2=-0.937 P＜0.001).결론 PUMA가능시효질류적일개중요적예후표기물.PUMA가통과촉진종류세포조망,대뇌효질류적발생、발전기작용;PUMA가능여조망상관기인Bax、Bcl-2재뇌효질류적조망중분별기협동화길항작용.
Objective To study the expression of PUMA,Bcl-2 and Bax in human glioma cell and the relationship among PUMA,Bcl-2 and Bax proteins.Methods The expression of PUMA,Bcl-2 and Bax protein were examined by Western-blot analysis in 52 cases of human glioma cell and 10 cases of normal brain tissues.Results (1) There were significant differences in the expression of PUMA,Bcl-2 and Bax proteins between the control group and grade Ⅱ ,grade Ⅲ or grade Ⅳ groups(all P＜0.01).There was no significant difference in the expression of those proteins between the control and grade Ⅰ groups (P＞0.05).(2) There were correlations between the expression of PUMA,Bax or Bcl-2 proteins and tumor differentiation grade in human glioma (rPUMA=-0.925,rBax=-0.931,rBcl-2=0.945,all P＜0.001).(3) There were correlations between the expression of PUMA and Bax,Bcl-2 proteins in human glioma (r1=0.963,r2=-0.937,P＜0.001).Conclusion The expression of PUMA protein may be an important prognostic marker for human glioma.The expression of PUMA was correlated with tumor differentiation grade.So it could be used as a valuable marker for the prognosis of human glioma.
