中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
10期
965-967
,共3页
视神经脊髓炎%Devic's病%NMO-IgG%水通道蛋白-4
視神經脊髓炎%Devic's病%NMO-IgG%水通道蛋白-4
시신경척수염%Devic's병%NMO-IgG%수통도단백-4
Neuromyelitis optica%Devic's disease%NMO-IgG%Aquaporin-4
目的 初步探讨以人工合成多肽模拟抗原酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测视神经脊髓炎(neuromyelitis optica,NMO)患者血清水通道蛋白-4(aquaporin-4,AQP4)抗体.方法根据AQP4蛋白的分子构成和三级结构,应用生物信息学和结构生物学的方法设计3段AQP4蛋白膜外段多肽片段,分别为AQP456-69、AQP4135-155、AQP4209-230;以其为抗原ELISA检测9例NMO及神经科其他疾病7例患者血清,并与免疫荧光法检测结果进行对比分析.结果 以AQP4135-155、AQP4209-230为抗原ELISA检测结果显示,9例NMO患者中,7例经免疫荧光法证实AQP4抗体阳性患者吸光度A值平均值比阴性对照患者显著提高(P＜0.05);当血清稀释4倍和8倍时具有较高的A值,与对照组相比差异具有统计学意义(P＜0.05).结论 AQP4蛋白2段膜外段多肽,即AQP4135-155和AQP4209-230可能是NMO患者血清中AQP4抗体所针对的主要抗原表位或抗原表位的主要部分,初步表明模拟抗原ELISA检测NMO患者血清AQP4抗体的可行性.
目的 初步探討以人工閤成多肽模擬抗原酶聯免疫吸附試驗(enzyme linked immunosorbent assay,ELISA)檢測視神經脊髓炎(neuromyelitis optica,NMO)患者血清水通道蛋白-4(aquaporin-4,AQP4)抗體.方法根據AQP4蛋白的分子構成和三級結構,應用生物信息學和結構生物學的方法設計3段AQP4蛋白膜外段多肽片段,分彆為AQP456-69、AQP4135-155、AQP4209-230;以其為抗原ELISA檢測9例NMO及神經科其他疾病7例患者血清,併與免疫熒光法檢測結果進行對比分析.結果 以AQP4135-155、AQP4209-230為抗原ELISA檢測結果顯示,9例NMO患者中,7例經免疫熒光法證實AQP4抗體暘性患者吸光度A值平均值比陰性對照患者顯著提高(P＜0.05);噹血清稀釋4倍和8倍時具有較高的A值,與對照組相比差異具有統計學意義(P＜0.05).結論 AQP4蛋白2段膜外段多肽,即AQP4135-155和AQP4209-230可能是NMO患者血清中AQP4抗體所針對的主要抗原錶位或抗原錶位的主要部分,初步錶明模擬抗原ELISA檢測NMO患者血清AQP4抗體的可行性.
목적 초보탐토이인공합성다태모의항원매련면역흡부시험(enzyme linked immunosorbent assay,ELISA)검측시신경척수염(neuromyelitis optica,NMO)환자혈청수통도단백-4(aquaporin-4,AQP4)항체.방법근거AQP4단백적분자구성화삼급결구,응용생물신식학화결구생물학적방법설계3단AQP4단백막외단다태편단,분별위AQP456-69、AQP4135-155、AQP4209-230;이기위항원ELISA검측9례NMO급신경과기타질병7례환자혈청,병여면역형광법검측결과진행대비분석.결과 이AQP4135-155、AQP4209-230위항원ELISA검측결과현시,9례NMO환자중,7례경면역형광법증실AQP4항체양성환자흡광도A치평균치비음성대조환자현저제고(P＜0.05);당혈청희석4배화8배시구유교고적A치,여대조조상비차이구유통계학의의(P＜0.05).결론 AQP4단백2단막외단다태,즉AQP4135-155화AQP4209-230가능시NMO환자혈청중AQP4항체소침대적주요항원표위혹항원표위적주요부분,초보표명모의항원ELISA검측NMO환자혈청AQP4항체적가행성.
Objective To explore mimic antigen based ELISA for detecting serum anti-acquaporin4(AQP4) antibody in neuromyelitis optica(NMO) patients. Methods Three polypeptides: AQP456-69,AQP4135-155, AQP4209-230 were designed to simulate antigen epitopes of AQP4 through biological information and structure biology analysis, the peptides was used as antigen in ELISA to detect serum anti-AQP4 antibody in 9 NMO patients and 7 other miscellaneous neurological disorders which hayed been detected by immumofluorescence method. Results The mean value of A of anti-AQP4 antibody postive patients which have been determined by immumofluorescence method were higher than the controls in ELISA with AQP4135-155,AQP4209-230 as antigen (P<0.05). When the patients serum were diluted at 4 and 8 times, the A values were higher than controls significantly ( P<0.05 ). Conclusion Outmembrane polypeptides AQP4135-155,AQP4209-230 may be the main antigen epitope or main part of antigen epitope, they could be used to detect serum anti-AQP4 antibody in NMO patients.
