CAJ | 학술논문

目的探讨1至4个月龄正常婴幼儿结膜囊的菌群分布及其相关影响因素.方法横断面研究方法.2009年1至3月天津医科大学眼科中心与天津市妇女儿童保健中心合作,采取简单随机抽样研究方法选择109例接受眼病筛查的1至4个月龄的健康小儿,进行双眼结膜囊分泌物取材并做细菌培养和鉴定.应用卡方检验、t检验和回归分析方法对数据进行统计学处理,分析结膜囊细菌种类和分布以及相关影响因素.结果结膜囊分泌物培养阳性的小儿26例(36只眼),占23.9%.共检出7种44株细菌,包括革兰阳性球菌25株,革兰阳性杆菌18株,革兰阴性杆菌1株.其中类白喉棒状杆菌17株(17/44,38.6%),表皮葡萄球菌16株(16/44,36.4%),金黄色葡萄球菌6株(6/44,13.6%).单眼分离出2种细菌者8只眼(8/36,22.2%),均为葡萄球菌和类白喉棒状杆菌.不同月龄、性别及喂养方式的细菌培养结果差异无统计学意义(X2=0.351,X2=0.001,X2=1.182,P值均大于0.05).结膜囊培养阳性者26例(36只眼),培养阴性者83例(166只眼),两组小儿的出生体重、胎龄及受检周龄比较差异无统计学意义(t=0.078,t=0.940,t=0.686,P值均大于0.05).本研究中性别、年龄、出生体重、胎龄及喂养方式不影响结膜囊带菌状态(Wald X2=0.001,Wald X2=0.003,Wald X2=0.117,Wald X2=1.307,Wald X2=1.490,P值均大于0.05).结论婴儿期结膜囊正常菌群已经建立,以表皮葡萄球菌和类白喉棒状杆菌为主要菌种.葡萄球菌与类白喉棒状杆菌有共生现象.小儿结膜囊带菌状态相关影响因素仍需进一步研究.
목적 탐토1지4개월령정상영유인결막낭적균군분포급기상관영향인소.방법 횡단면연구방법.2009년1지3월천진의과대학안과중심여천진시부녀인동보건중심합작,채취간단수궤추양연구방법선택109례접수안병사사적1지4개월령적건강소인,진행쌍안결막낭분비물취재병주세균배양화감정.응용잡방검험、t검험화회귀분석방법대수거진행통계학처리,분석결막낭세균충류화분포이급상관영향인소.결과 결막낭분비물배양양성적소인26례(36지안),점23.9%.공검출7충44주세균,포괄혁란양성구균25주,혁란양성간균18주,혁란음성간균1주.기중류백후봉상간균17주(17/44,38.6%),표피포도구균16주(16/44,36.4%),금황색포도구균6주(6/44,13.6%).단안분리출2충세균자8지안(8/36,22.2%),균위포도구균화류백후봉상간균.불동월령、성별급위양방식적세균배양결과차이무통계학의의(X2=0.351,X2=0.001,X2=1.182,P치균대우0.05).결막낭배양양성자26례(36지안),배양음성자83례(166지안),량조소인적출생체중、태령급수검주령비교차이무통계학의의(t=0.078,t=0.940,t=0.686,P치균대우0.05).본연구중성별、년령、출생체중、태령급위양방식불영향결막낭대균상태(Wald X2=0.001,Wald X2=0.003,Wald X2=0.117,Wald X2=1.307,Wald X2=1.490,P치균대우0.05).결론 영인기결막낭정상균군이경건립,이표피포도구균화류백후봉상간균위주요균충.포도구균여류백후봉상간균유공생현상.소인결막낭대균상태상관영향인소잉수진일보연구.
Objective To investigate the normal conjunetival bacterial flora isolated from healthy infants aged from 1 to 4 months and to analyze its correlated factors.Methods This was a cross-sectional study.From January to March 2009,109 cases of healthy babies aged from 1 to 4 months were selected throulgh simple random sampling when they accepted health examination provided by Tianjin Medical University Eye Center and Tianjin Women and Children's Health Center.Conjunctival swabs were taken from both eyes and inoculated onto blood agar plates.The isolated bacteria were classified professionally.The distribution of different bacteria was investigated as well as the possible correlated factors by using t-test,X2 test and binary logistic regression of SPSS 11.5.Results 26 cases of healthy babies(23.9%)got positive culture results which consisted 44 strains of ocular bacterial isolates.25 strains were gram-positive cocci.while 18 strains gram-positive bacilli,and 1 strain gram-negative bacillus.The first three isolated bacteria were Diphthemid bacillus(17/44,38.6%),Staphylococcus epidermitis(16/44,36.4%),and Staphylococcus aureus(6/44,13.6%).The two subtypes of Staphylococci coexisted with Diphthemid bacillus in 8 eyes of 36 eyes from which bacteria were isolated.The bacterial isolation rate through every month age group had no significant difference(X2=0.351,P>0.05).There wag no significant difference in the group of positive isolation(26 cases,36 eyes)or negative isolation(83 cases,166 eyes)through breast,formula or mixed feeding(X2=1.182,P>0.05).The gender had no influence on the detection of bacteria(X2=0.001,P>0.05).The birth weight,gestational age or examined age between the two groups had no significant difference (t=0. 078, t=0. 940, t=0. 686,P>0. 05). The gender, age, birth weight,gestational age and the way of feeding had no correlation with existence of conjunctival flora in these healthy infants ( Wald X2 = 0. 001, Wald X2 = 0. 003, Wald X2 = 0. 117, Wald X2 = 1. 307, Wald X2 = 1. 490, P >0. 05). Conclusions The normal conjunetival flora might establish during the initial months of little babies. Staphylococcus epidermitis and Diphthemid bacillus are the most common bacteria. Future studies should be conducted to make sure whether there would be some factors correlated with the culture results.