CAJ | 학술논문

目的探讨人骨髓间充质干细胞( MSC)中血红素加氧酶1(HO-1)的表达及HO-1对MSC凋亡的影响.方法体外培养人骨髓MSC,反转录PCR (RT-PCR)检测人骨髓MSC中HO-1mRNA的表达；MSC加入HO-1特异性抑制剂锌原卟啉Ⅸ(Znpp -Ⅸ)抑制HO-1后,采用形态学观察、流式细胞术检测MSC的凋亡变化.结果人骨髓MSC中有HO-1 mRNA的表达,加入HO-1特异性抑制剂Znpp-Ⅸ12 h后,倒置显微镜下观察可见MSC细胞开始出现不规则改变且胞体缩小,胞膜皱缩；凋亡细胞(Annexin V+/PI -)百分数高于对照MSC(61.21％ ±5.28％比7.48％±0.69％,P ＜0.05)；细胞周期中在Go/G1峰前出现1个明显的凋亡细胞峰,凋亡细胞数高于对照MSC(21.36％ ±0.49％比1.02％±0.05％,P＜0.05).结论 HO-1在人骨髓MSC中表达并发挥抗细胞凋亡的作用.
목적 탐토인골수간충질간세포( MSC)중혈홍소가양매1(HO-1)적표체급HO-1대MSC조망적영향.방법 체외배양인골수MSC,반전록PCR (RT-PCR)검측인골수MSC중HO-1mRNA적표체；MSC가입HO-1특이성억제제자원계람Ⅸ(Znpp -Ⅸ)억제HO-1후,채용형태학관찰、류식세포술검측MSC적조망변화.결과 인골수MSC중유HO-1 mRNA적표체,가입HO-1특이성억제제Znpp-Ⅸ12 h후,도치현미경하관찰가견MSC세포개시출현불규칙개변차포체축소,포막추축；조망세포(Annexin V+/PI -)백분수고우대조MSC(61.21％ ±5.28％비7.48％±0.69％,P ＜0.05)；세포주기중재Go/G1봉전출현1개명현적조망세포봉,조망세포수고우대조MSC(21.36％ ±0.49％비1.02％±0.05％,P＜0.05).결론 HO-1재인골수MSC중표체병발휘항세포조망적작용.
Objective To explore the role of heme oxygenase-1 (HO-1) in the apoptosis of mesenchymal stem cells (MSCs).Methods The method of reverse transcription PCR (RT-PCR) was employed to detect the expression of HO-1 mRNA in bone marrow MSCs. Morphologic observations,flow cytometry Annexin-V and DNA analysis were used to detect apoptosis and determine the content of DNA of MSCs after a 12-hour treatment of Znpp-Ⅸ,a Ho-1 special inhibitor.Results HO-1 mRNA was expressed in normal bone marrow MSCs. After culturing Znpp-Ⅸ for 12 h,MSCs showed volume reduction and membrane shrinkage.The cells of apoptotic Annexin V +/PI- increased significantly (61.21％ ± 5.28％ vs 7.48％ ± 0.69％,P ＜ 0.05 ). The peak of apoptosis was distinct on the distribution graph of DNA (21.36％ ±0.49％ vs 1.02％ ±0.05％,P ＜0.05).Conclusion HO-1 plays an anti-apoptotic role in human bone marrow mesenchymal stem cells.