CAJ | 학술논문

目的通过分离、鉴定胆囊癌和胆囊良性组织的差异表达蛋白质,探讨可能用于早期诊断或治疗胆囊癌的肿瘤标志物.方法对6例人胆囊癌组织和胆囊良性组织进行比较蛋白质组学研究,采用双向凝胶电泳分离蛋白,经图像分析识别差异表达的蛋白.应用基质辅助激光解析电离飞行时间质谱(MALD-TOF-MS)鉴定差异蛋白质.采用免疫组化技术对差异蛋白磷脂酰乙醇胺结合蛋白1(PEBP1)进行验证.结果获得了分辨率和重复性均较好的凝胶蛋白图谱.共筛选出46个在胆囊癌组织中明显差异表达的蛋白点,其中17个蛋白质鉴定成功.在这17个蛋白质中,胆囊癌组织中高表达9个,低表达8个.PEBP1在胆囊癌组织中的阳性表达率为74.0%.结论成功鉴定了17个胆囊癌相关蛋白,为进一步筛选胆囊癌的诊断、治疗和预后评估的分子标志物奠定了基础.
목적 통과분리、감정담낭암화담낭량성조직적차이표체단백질,탐토가능용우조기진단혹치료담낭암적종류표지물.방법 대6례인담낭암조직화담낭량성조직진행비교단백질조학연구,채용쌍향응효전영분리단백,경도상분석식별차이표체적단백.응용기질보조격광해석전리비행시간질보(MALD-TOF-MS)감정차이단백질.채용면역조화기술대차이단백린지선을순알결합단백1(PEBP1)진행험증.결과 획득료분변솔화중복성균교호적응효단백도보.공사선출46개재담낭암조직중명현차이표체적단백점,기중17개단백질감정성공.재저17개단백질중,담낭암조직중고표체9개,저표체8개.PEBP1재담낭암조직중적양성표체솔위74.0%.결론 성공감정료17개담낭암상관단백,위진일보사선담낭암적진단、치료화예후평고적분자표지물전정료기출.
Objective To find out potential molecular targets for gallbladder carcinoma diagnosis and treatment by analyzing and comparing the proteins expressed in human gallbladder carcinoma tissue and benign gallbladder tissue. Methods Proteomic analysis of 6 human gallbladder carcinoma tissues and 6 benign gallbladder tissues was carried out. Total proteins of the carcinoma tissue and benign gallbladder tissue were separated by two-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were analyzed and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALD1-TOF-MS). Immunohistochemistry was used to examine the expression of PEBP1 protein in an independent series of samples. Results Protein extracts of individual samples in each type of tissues were separated on two-dimensional gels. There were forty six differentially expressed proteins in the gallbladder carcinom tissues. Seventeen proteins were successfully identified by MS, in which nine proteins were overexpressed in tumors while the other eight proteins were underexpressed. The increased level of PEBP1 protein in gallbladder carcinoma was further confirmed by immunohistochemical analysis. Conclusion Seventeen differentially expressed proteins were successfully characterized by comparative proteomic analysis. Those results may provide scientific foundation for screening the molecular biomarkers which can be used in diagnosis and treatment of gallbladder carcinoma, as well as to improve its prognosis and provide a new clue for carcinogenesis research of gallbladder carcinoma.