CAJ | 학술논문

用毛细管电泳法对5种常用的抗生素四环素(TC),土霉素(OTC),多西环素(DOC),金霉素(CTC)和氯霉素(CP)进行了分离。研究了电泳缓冲液的pH、不同有机试剂添加剂、温度等因素对抗生素分离的影响。实验结果表明，体积分数为4%的N-甲基吗啡啉的加入可大大改善分离效果。在各自相应的浓度范围内，峰面积和样品浓度之间呈现良好的线性关系，重现性好。氯霉素的检测极限为10μg/L,四环素、土霉素、多西环素为20μg/L，金霉素为40μg/L(信噪比>5)。该方法成功地应用于蜂蜜中残留抗生素的测定，具有操作简单、快速方便、灵敏度及自动化程度高、重现性好等优点，是对含抗生素类物质进行痕量分析的好方法。
용모세관전영법대5충상용적항생소사배소(TC),토매소(OTC),다서배소(DOC),금매소(CTC)화록매소(CP)진행료분리。연구료전영완충액적pH、불동유궤시제첨가제、온도등인소대항생소분리적영향。실험결과표명，체적분수위4%적N-갑기마배람적가입가대대개선분리효과。재각자상응적농도범위내，봉면적화양품농도지간정현량호적선성관계，중현성호。록매소적검측겁한위10μg/L,사배소、토매소、다서배소위20μg/L，금매소위40μg/L(신조비>5)。해방법성공지응용우봉밀중잔류항생소적측정，구유조작간단、쾌속방편、령민도급자동화정도고、중현성호등우점，시대함항생소류물질진행흔량분석적호방법。
Five antibiotics compounds, tetracycline(TC), oxytetracycline(OTC), doxyc ycline(DOC), chlortetracycline(CTC) and chloramphenicol(CP), were successfull y separa ted and determined by high performance capillary electrophoresis(HPCE). Effects of buffer pH, various organic additives and temperature on electrophoretic separ ation of antibiotics were investigated. Satisfactory separation of these five a n tibiotics was achieved in the buffer of pH 3.2, 0.02 mol/L Na2HPO4-0.01 mol/L citric acid w ith addition of 4% (V/V) N-methylmorpholine and 12% (V/V) acetonitrile within 20 minutes. Th e calibration graphs were linear by plotting the peak area against the sample concen tration over the range of 150 μg/L to 750 μg/L and the correlat ion coeffic ients were greater than (0.991 7). The detection limits were 10 μg/L for C P, 20 μg/L for TC, OTC and DOC and 40 μg/L for CP (signal to noise ratio>5). HPCE method was successfully applied to the analysis of trace antibiotics in honey.