CAJ | 학술논문

背景:原花青素是一种葡萄籽提取物,具抗氧化清除自由基的作用.研究发现运动引起骨骼肌自由基和其他它形式的活性氧增加是导致骨骼肌损伤和疲劳的重要原因之一.补充抗氧化剂能阻抑这种改变,提高运动能力. 目的:研究葡萄籽提取物原花青素的抗疲劳作用. 设计:以实验动物为研究对象,完全随机设计,对照实验研究. 单位:省级疾病预防控制中心毒理室. 材料:实验于 2001- 09/12在江苏省疾病预防控制中心毒理室完成.采用雄性昆明种小鼠,普通级,购自中国药科大学实验动物中心.动物合格证号: SCXK(苏 ) 2002-0011;实验动物环境设施合格证号: SYXK(苏) 2001-0004号.按体质量随机分为溶剂对照组和 3个实验组,即原花青素低、中、高剂量组,每组 10只,共 120只.实验组每天经口给予原花青素.方法: 3个实验组分别经口给与原花青素 1.7, 16.7, 50 mg/kg体质量,对照组给与双蒸水,连续 30 d.给与原花青素 30 d后测定小鼠负重游泳时间,肝糖元含量,血乳酸及血红蛋白含量. 主要观察指标 :小鼠负重游泳时间,肝糖元含量,血乳酸及血红蛋白含量. 结果:各剂量组小鼠负重游泳时间均比对照组的延长,中、高剂量组分别达到 (17.84± 8.48) min和 (25.80± 7.45) min,差异具极显著意义 (P< 0.01),各剂量组小鼠运动后血乳酸含量均低于对照组,高剂量组 (6.78± 2.45) mmol/L与对照组 (9.98± 1.22) mmol/L)相比差异具极显著意义 (P< 0.01);肝糖元含量均高于对照组, 中剂量组 [(1 244.65 ± 177.58)mg/100 g 肝 ],高剂量组 [(1 383.96± 141.20) mg/100 g 肝 ]与对照组 [(817.67± 114.72) mg/100 g 肝 ]相比差异分别具显著和非常显著性意义 (P< 0.05,P< 0.01) . 结论 :原花青素具有抗疲劳作用.
배경:원화청소시일충포도자제취물,구항양화청제자유기적작용.연구발현운동인기골격기자유기화기타타형식적활성양증가시도치골격기손상화피로적중요원인지일.보충항양화제능조억저충개변,제고운동능력. 목적:연구포도자제취물원화청소적항피노작용. 설계:이실험동물위연구대상,완전수궤설계,대조실험연구. 단위:성급질병예방공제중심독리실. 재료:실험우 2001- 09/12재강소성질병예방공제중심독리실완성.채용웅성곤명충소서,보통급,구자중국약과대학실험동물중심.동물합격증호: SCXK(소 ) 2002-0011;실험동물배경설시합격증호: SYXK(소) 2001-0004호.안체질량수궤분위용제대조조화 3개실험조,즉원화청소저、중、고제량조,매조 10지,공 120지.실험조매천경구급여원화청소.방법: 3개실험조분별경구급여원화청소 1.7, 16.7, 50 mg/kg체질량,대조조급여쌍증수,련속 30 d.급여원화청소 30 d후측정소서부중유영시간,간당원함량,혈유산급혈홍단백함량. 주요관찰지표 :소서부중유영시간,간당원함량,혈유산급혈홍단백함량. 결과:각제량조소서부중유영시간균비대조조적연장,중、고제량조분별체도 (17.84± 8.48) min화 (25.80± 7.45) min,차이구겁현저의의 (P< 0.01),각제량조소서운동후혈유산함량균저우대조조,고제량조 (6.78± 2.45) mmol/L여대조조 (9.98± 1.22) mmol/L)상비차이구겁현저의의 (P< 0.01);간당원함량균고우대조조, 중제량조 [(1 244.65 ± 177.58)mg/100 g 간 ],고제량조 [(1 383.96± 141.20) mg/100 g 간 ]여대조조 [(817.67± 114.72) mg/100 g 간 ]상비차이분별구현저화비상현저성의의 (P< 0.05,P< 0.01) . 결론 :원화청소구유항피노작용.
BACKGROUND:Proanthocyanidin,a kind of grape seed extract(GSE),has the effect of anti-oxidation and elimination of free radicals.Research finds that increase of free radicals in skeletal muscle and active oxygen in other forms induced by exercise is one of the important causes of the injury and fatigue of skeletal muscle. Supplement of antioxidant can inhibit this kind of change and improve motor ability. OBJECTIVE:To study the anti-fatigue effects of proanthocyanidin. DESIGN:A completely randomized controlled experimental study taking experimental animals as the subjects. SETTING:A toxicological department of a provincial center for disease control and prevention. PARTICIPANTS:The experiment was completed in the Department of Toxicology,Jiangsu Center for Disease Control and Prevention from September to December 2001.Male mice of Kunming strain and common grade[certification number:SCXK(Su) 2002-0011;certification number of environmental equipment for experimental animal:SYXK(Su) 2001-0004]were purchased from the Experiment Animal Center of Chinese Pharmacological University.The 120 mice were randomly divided according to body mass into control group and 3 experiment groups, namely,low-,medium-and high-dose GSE groups,with 10 mice in each group.The mice in the 3 experiment groups received oral administration of GSE every day. INTERVENTIONS:The mice in the 3 experiment groups received oral administration of GSE of 1.7, 16.7 and 50.0 mg/kg body mass,respectively,while those in control group were only given twice distilled water for 30 consecutive days. After GSE treatment for 30 days,the loaded swimming time, contents of hepatic glycogen,blood lactic acid(LA) and hemoglobin were measured. MAIN OUTCOME MEASURES:Loaded swimming time, hepatic glycogen,LA and hemoglobin contents. RESULTS:The loaded swimming time of mice in experiment groups was significantly prolonged as compared with that in control group (P< 0.01),with(17.84± 8.48) and(25.80± 7.45) minutes in medium-and high-dose GSE groups,respectively.LA content after exercise in experiment groups was all lower than that in control group,with greatly significant difference between high-dose GSE group[(6.78± 2.45) mmol/L] and control group[(9.98± 1.22) mmol/L](P< 0.01).The content of hepatic glycogen after exercise in experiment groups was higher than that in control group; medium-dose GSE group[(1 244.65(177.58) mg/100 g liver mass]and high-dose GSE group[(1 383.96± 141.20) mg/100 g liver mass]differed greatly significantly from control group[(817.67(114.72) mg/100 g liver mass](P< 0.05,P< 0.01). CONCLUSION:Grape seed extract proanthocyanidin has an anti-fatigue effect.