辐射研究与辐射工艺学报
輻射研究與輻射工藝學報
복사연구여복사공예학보
JOURNAL OF RADIATION RESEARCH AND RADIATION PROCESSING
2008年
1期
33-38
,共6页
徐新萍%彭瑞云%高亚兵%陈浩宇%崔雪梅%王德文%陈隈陟%董波%杨怡%邱萍
徐新萍%彭瑞雲%高亞兵%陳浩宇%崔雪梅%王德文%陳隈陟%董波%楊怡%邱萍
서신평%팽서운%고아병%진호우%최설매%왕덕문%진외척%동파%양이%구평
骨髓%造血细胞%中子%γ射线%死亡方式
骨髓%造血細胞%中子%γ射線%死亡方式
골수%조혈세포%중자%γ사선%사망방식
Bone marrow%Hematopoietic cells%Neutron%γ-rays%Death mode
为探讨中子和γ射线照射后小鼠骨髓造血细胞的死亡方式及其意义,采用不同剂量中子和60Co γ射线全身照射350只BALB/c小鼠,通过光镜、电镜、流式细胞术、原位末端标记和DNA凝胶电泳等观察造血细胞凋亡与坏死情况.研究发现,中子照射后小鼠骨髓中坏死和凋亡的造血细胞均明显增多,5.5Gy组以坏死为主,而γ射线照射后则以凋亡为主,且呈剂量相关性.凋亡的细胞表现为核染色质浓缩、边移,呈半月型、环状或不规则状,凋亡小体形成,电泳下见DNA梯状图谱.坏死的细胞表现为核肿胀、溶解,线粒体膜、核膜等膜性结构破坏.2.5-5.5Gy中子及5.5-12Gyγ射线照射可使骨髓造血细胞死亡方式不同:中子照射以凋亡与坏死并存,5.5Gy组以坏死为主;而γ射线照射则以凋亡为主.
為探討中子和γ射線照射後小鼠骨髓造血細胞的死亡方式及其意義,採用不同劑量中子和60Co γ射線全身照射350隻BALB/c小鼠,通過光鏡、電鏡、流式細胞術、原位末耑標記和DNA凝膠電泳等觀察造血細胞凋亡與壞死情況.研究髮現,中子照射後小鼠骨髓中壞死和凋亡的造血細胞均明顯增多,5.5Gy組以壞死為主,而γ射線照射後則以凋亡為主,且呈劑量相關性.凋亡的細胞錶現為覈染色質濃縮、邊移,呈半月型、環狀或不規則狀,凋亡小體形成,電泳下見DNA梯狀圖譜.壞死的細胞錶現為覈腫脹、溶解,線粒體膜、覈膜等膜性結構破壞.2.5-5.5Gy中子及5.5-12Gyγ射線照射可使骨髓造血細胞死亡方式不同:中子照射以凋亡與壞死併存,5.5Gy組以壞死為主;而γ射線照射則以凋亡為主.
위탐토중자화γ사선조사후소서골수조혈세포적사망방식급기의의,채용불동제량중자화60Co γ사선전신조사350지BALB/c소서,통과광경、전경、류식세포술、원위말단표기화DNA응효전영등관찰조혈세포조망여배사정황.연구발현,중자조사후소서골수중배사화조망적조혈세포균명현증다,5.5Gy조이배사위주,이γ사선조사후칙이조망위주,차정제량상관성.조망적세포표현위핵염색질농축、변이,정반월형、배상혹불규칙상,조망소체형성,전영하견DNA제상도보.배사적세포표현위핵종창、용해,선립체막、핵막등막성결구파배.2.5-5.5Gy중자급5.5-12Gyγ사선조사가사골수조혈세포사망방식불동:중자조사이조망여배사병존,5.5Gy조이배사위주;이γ사선조사칙이조망위주.
In order to investigate the death mode of hematopoictic cells in mouse bone marrow irradiated by neutron and γ-rays, 350 BALB/C mice, which were whole body irradiated with varies doses of neutron and γ-rays,were sacrificed at 6 and 12bours, 1, 2, 3, 4, 5, 7, 10, 14, 21 and 28days after irradiation respectively. By means of light microscope, electron microscope, flow cytometry, in situ terminal end labeling and DAN gel electrophoresis, the apoptosis and necrosis of hematopoietic cells were investigated. The results showed that the amount of hematopoietic cells occurring apoptosis and necrosis were obviously increased after neutron irradiation and the necrosis was main death mode in irradiation dose of 5.5Gy. Meanwhile, γ-irradiation mainly induced apoptosis of hematopoietic cells and the apoptosis was correlated to the irradiation dose. It means that there were different death modes of hematopoietic cells in mouse bone marrow irradiated by neutron and γ-rays. Neutron mainly induced necrosis of the hematopoietic cells though apoptosis also occurred in irradiation dose of 5.5Gy, while γ-rays mainly induced the apoptosis.
