CAJ | 학술논문

目的建立一种香草醛-硫酸比色法测定桔梗中总皂苷含量的方法,为桔梗的质量控制提供科学依据.方法索氏提取器提取,大孔吸附树脂纯化,香草醛-硫酸比色法测定桔梗总皂苷含量.结果比色法测定的线性方程为A=0.003 4C+0.006 4,相关系数为r=0.999 7,平均加样回收率为96.97%,RSD=1.51%(n=6).对不同来源桔梗中总皂苷含量进行测定,其含量范围为1.07%～3.76%.结论该法测定准确,重复性好,可用于桔梗药材中桔梗总皂苷的含量测定,便于对桔梗药材进行质量评价.
목적 건립일충향초철-류산비색법측정길경중총조감함량적방법,위길경적질량공제제공과학의거.방법 색씨제취기제취,대공흡부수지순화,향초철-류산비색법측정길경총조감함량.결과 비색법측정적선성방정위A=0.003 4C+0.006 4,상관계수위r=0.999 7,평균가양회수솔위96.97%,RSD=1.51%(n=6).대불동래원길경중총조감함량진행측정,기함량범위위1.07%～3.76%.결론 해법측정준학,중복성호,가용우길경약재중길경총조감적함량측정,편우대길경약재진행질량평개.
Objective To develop a vanillin -H2SO4 colorimetry method for the determination of total saponins in Radix Platycodonis samples,in order to provide a scientific basis for the quality control of Radix Platycodonis.Methods With Platycodin D as standard,the total saponins in Radix platycodonis was extracted with soxhlet extractor and purified with macroporous resin column chromatography,its content was determined by vanillin -H2SO4 colorimetry method.Results The calibration curve of total saponins in Radix Platycodonis samples for this method was A=0.003 4C+0.006 4,r=0.999 7.The average recovery and RSD were 96.97% and 1.51%,respectively.The content range of total saponins in Radix platycodonis from different origins was from 1.07% to 3.76%.Conclusion This determination method is practical,reproducible and can be used for evaluating the quality of Radix Platycodonis samples.