CAJ | 학술논문

奶牛乳腺上皮细胞具有合成和分泌乳汁的特殊功能,是制作乳腺生物反应器的靶细胞.以奶牛乳腺上皮细胞为试验模型,能够真实反应乳腺生长、发育及泌乳的细胞或分子生物学机制,验证乳腺组织特异性表达载体构建的合理性和有效性,对研究奶牛乳腺生物反应器、乳蛋白基因表达具有重要的意义.文章主要从培养方法、培养体系的建立、形态结构与细胞增殖分化方面阐述乳腺上皮细胞体外培养技术研究进展.
내우유선상피세포구유합성화분비유즙적특수공능,시제작유선생물반응기적파세포.이내우유선상피세포위시험모형,능구진실반응유선생장、발육급비유적세포혹분자생물학궤제,험증유선조직특이성표체재체구건적합이성화유효성,대연구내우유선생물반응기、유단백기인표체구유중요적의의.문장주요종배양방법、배양체계적건립、형태결구여세포증식분화방면천술유선상피세포체외배양기술연구진전.
Diary cow mammary gland epithelial cells have special functions of synthesis and lactation, and make manufacture mammary gland bioreactor harrow cell. Diary cow mammary gland epithelial cells for experimental model could actually response mammoplasia, development, lactotroph or molecular biology mechanism, and validate tissue specificity expression vector rationality and effectivity. It has to study and research diary cow mammary gland bioreactor lactoprotein gene expression the siguificece. This paper major introduced cultural method, cultural system, morphosis, cell proliferation and differentiation aspects to explain the research progress of culture technique on mammary gland epithelial cells in vitro.