扬州大学学报（农业与生命科学版）
颺州大學學報（農業與生命科學版）
양주대학학보（농업여생명과학판）
JOURNAL OF YANGZHOU UNIVERSITY(AGRICULTURAL AND LIFE SCIENCE EDITION)
2009年
4期
105-110
,共6页
宋东杰%华春%李朝晖%陈全战%周泉澄%王小平%杜开河%秦春
宋東傑%華春%李朝暉%陳全戰%週泉澄%王小平%杜開河%秦春
송동걸%화춘%리조휘%진전전%주천징%왕소평%두개하%진춘
伊乐藻%As~(3+)%Se~(4+)%叶绿素%可溶性蛋白%抗氧化保护酶系统%亚显微结构
伊樂藻%As~(3+)%Se~(4+)%葉綠素%可溶性蛋白%抗氧化保護酶繫統%亞顯微結構
이악조%As~(3+)%Se~(4+)%협록소%가용성단백%항양화보호매계통%아현미결구
Elodea nuttalli%As~(3+)%Se~(4+)%chlorophyll%soluble protein%antioxidant enzyme system%submicroscopic structure
以伊乐藻为试验材料,研究砷、砷+硒离子对伊乐藻抗氧化酶系统的影响.研究表明:As~(3+)对伊乐藻的急性毒害主要表现为叶绿素、可溶性蛋白的含量随处理时间和浓度的增加呈逐步下降趋势;抗氧化保护酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)]活性随处理时间和浓度的变化而变化,SOD活性呈先升后降、POD活性至第6天升至高点后下降、CAT活件则随时间延长呈上升趋势.亚显微结构观察显示,叶绿体、线粒体遭到破坏.Se~(4+)在较低浓度时对As~(3+)的毒害有一定的拮抗保护作用.
以伊樂藻為試驗材料,研究砷、砷+硒離子對伊樂藻抗氧化酶繫統的影響.研究錶明:As~(3+)對伊樂藻的急性毒害主要錶現為葉綠素、可溶性蛋白的含量隨處理時間和濃度的增加呈逐步下降趨勢;抗氧化保護酶[超氧化物歧化酶(SOD)、過氧化物酶(POD)、過氧化氫酶(CAT)]活性隨處理時間和濃度的變化而變化,SOD活性呈先升後降、POD活性至第6天升至高點後下降、CAT活件則隨時間延長呈上升趨勢.亞顯微結構觀察顯示,葉綠體、線粒體遭到破壞.Se~(4+)在較低濃度時對As~(3+)的毒害有一定的拮抗保護作用.
이이악조위시험재료,연구신、신+서리자대이악조항양화매계통적영향.연구표명:As~(3+)대이악조적급성독해주요표현위협록소、가용성단백적함량수처리시간화농도적증가정축보하강추세;항양화보호매[초양화물기화매(SOD)、과양화물매(POD)、과양화경매(CAT)]활성수처리시간화농도적변화이변화,SOD활성정선승후강、POD활성지제6천승지고점후하강、CAT활건칙수시간연장정상승추세.아현미결구관찰현시,협록체、선립체조도파배.Se~(4+)재교저농도시대As~(3+)적독해유일정적길항보호작용.
The toxicity of As~(3+) and As~(3+) +Se~(4+) to Elodea nuttalli was studied. The results showed that As~(3+) had acute toxicity. The content of chlorophyll and soluble proteins tended to decrease. And the antioxident enzyme system [-superoxide dismutase (SOD), peroxidese (POD), catalase (CAT)]varied as treatment time and concentration. The con-tent of SOD increased at early stage of treatment but decreased at the late stage. The activity of POD was highest at six days of treatment. After then it decreased. The activity of CAT increased with treatment time. Submicroscopic structure revealed that the structure of chloroplasts and mitochondria was damaged. While lower concentration of Se~(4+) was added, the toxicity of As~(3+) was reduced.
