CAJ | 학술논문

枯草芽孢杆菌(Bacillus subtilis)LL18-4菌株分泌物中的类细菌素通过硫酸铵盐析法粗提后,经过Sephadex G-75、DEAE-Sephadex A 50和Sephacryl S-200HR分步柱层析,SDS-PAGE电泳检测到单一蛋白质条带,分子量约为60 kD. 该类细菌素在酸性条件下及80 %饱和度的硫酸铵溶液中具有较高的稳定性,可4 ℃放置3个月仍保持80 %以上活性;对有机溶剂有一定的耐受性;Mg~(2+) 能提高其抑菌活性. 指数增长前期添加柑桔绿霉 (Peniicilbum diigitatum) 病原真菌的培养液可以提高该类细菌素的分泌量.
고초아포간균(Bacillus subtilis)LL18-4균주분비물중적류세균소통과류산안염석법조제후,경과Sephadex G-75、DEAE-Sephadex A 50화Sephacryl S-200HR분보주층석,SDS-PAGE전영검측도단일단백질조대,분자량약위60 kD. 해류세균소재산성조건하급80 %포화도적류산안용액중구유교고적은정성,가4 ℃방치3개월잉보지80 %이상활성;대유궤용제유일정적내수성;Mg~(2+) 능제고기억균활성. 지수증장전기첨가감길록매 (Peniicilbum diigitatum) 병원진균적배양액가이제고해류세균소적분비량.
A bacteriocin-like substance(BLS) produced by Bacillus subtilis LL18-4 was purified via ammonnium sulphate precipitation, Sephadex G-75, DEAE-Sephadex A 50 and Sepharyl S-200 HR chromatography, and in the final purification step, one active fraction was obtained and the molecular weight of the purified BLS determined by SDS-PAGE was about 60 kD. The BLS was able to resist the denaturation action of several organic solvents and the antifungal activity was increased when incubated with Mg2+. The fermentation ability of LL18-4 was improved when the fermentation of Peniicilbum diigitatum was added in the early exponential growth phase.