CAJ | 학술논문

采用10 nm的纳米金标记羊抗人免疫球蛋白G获得免疫球蛋白G (IgG)的探针(AuIgG). 在pH 6.8的NaH_2PO_4-Na_2HPO_4磷酸盐缓冲溶液及聚乙二醇6000、 KCl溶液存在下, IgG与AuIgG探针发生免疫反应, 用0.15 μm滤膜过滤反应生成的免疫复合物溶液, 滤液在524 nm处有一最大吸收峰. 其降低值ΔA_(524 nm)随着IgG浓度的增加线性增加, 据此建立了一种测定IgG的分光光度法. 在最佳实验条件下, 免疫球蛋白G浓度在0.025～0.375 μg/mL范围内与ΔA呈良好的线性关系, 其线性回归方程为ΔA=0.783ρ+0.0232, 相关系数为0.9927, 检出限(3σ)为0.0082 μg/mL. 该法用于分析人血清中免疫球蛋白G, 结果与免疫透射比浊法结果一致, 相对标准偏差在2.0%～5.6%之间.
채용10 nm적납미금표기양항인면역구단백G획득면역구단백G (IgG)적탐침(AuIgG). 재pH 6.8적NaH_2PO_4-Na_2HPO_4린산염완충용액급취을이순6000、 KCl용액존재하, IgG여AuIgG탐침발생면역반응, 용0.15 μm려막과려반응생성적면역복합물용액, 려액재524 nm처유일최대흡수봉. 기강저치ΔA_(524 nm)수착IgG농도적증가선성증가, 거차건립료일충측정IgG적분광광도법. 재최가실험조건하, 면역구단백G농도재0.025～0.375 μg/mL범위내여ΔA정량호적선성관계, 기선성회귀방정위ΔA=0.783ρ+0.0232, 상관계수위0.9927, 검출한(3σ)위0.0082 μg/mL. 해법용우분석인혈청중면역구단백G, 결과여면역투사비탁법결과일치, 상대표준편차재2.0%～5.6%지간.
Nanogold particles of 10 nm were used to label goat anti-human IgG (GIgG) to obtain nanogold-labeled IgG (AuIgG). In the pH 6.8 NaH_2PO_4-Na_2HPO_4 buffer solution and in the presence of polyethylene glycol (PEG) 6000 and KCl, AuGIgG combined with IgG and aggregated to form immunocomplex AuIgG-IgG particles by Val der Waals force, hydrophobic force, coulomb attracting force and hydrogen bond binding force, and nanogold particles form larger nanogold clusters that can be removed by membrane filtration. With addition of IgG, the decreased ΔA value of solution increased linearly. Thus, a new spectrophotometric method was proposed. Under the chosen conditions, the decreased ΔA value at 524 nm was proportional to the concentration of IgG in the range of 0.025～0.375 μg/mL, the regression equation was ΔA_(524 nm)=0.783ρ+0.0232, and correlation coefficient was 0.9927, with a detection limit of 0.0082 μg/mL. This assay was applied to the analysis of IgG in human sera, which was also determined by the immunoturbidimetric method, and both results were consistent. The RSDs were 2.0%～5.6%. The method has high sensitivity, high selectivity and rapidity.