CAJ | 학술논문

目的探讨参附注射液(SF)单次预处理对大鼠脑缺血-再灌注损伤的保护作用.方法 30只雄性SD大鼠随机均分为单纯缺血-再灌注组(MCAO组)、SF预处理组(SF组,缺血前30 min经腹腔注射SF 10 ml/kg)和假手术组(Sham组).MCAO组和SF组采用颈内动脉尼龙线线栓法致右侧大脑中动脉栓塞120 min.观察再灌注后24 h时脑组织病理学改变,检测热休克蛋白(HSP)70和HSP90表达.结果再灌注24 h后,SF组脑组织病理学损害轻于MCAO组.Sham组未见HSP70及HSP90表达.MCAO组可见到较多的HSP70免疫阳性细胞.明显高于Sham组(P<0.01);SF组HSP70免疫阳性细胞分布较MCAO组广泛,数量明显增多,胞浆及胞核都可见HSP70免疫阳性细胞[(1.341±0.464)vs.(0.856±0.574)](P<0.05),而SF组HSP90的表达与MCAO组相仿(0.006±0.013)vs.(0.005±0.007)].结论 SF预处理可通过增加HSP70表达而对大鼠脑缺血-再灌注损伤起保护作用.
목적 탐토삼부주사액(SF)단차예처리대대서뇌결혈-재관주손상적보호작용.방법 30지웅성SD대서수궤균분위단순결혈-재관주조(MCAO조)、SF예처리조(SF조,결혈전30 min경복강주사SF 10 ml/kg)화가수술조(Sham조).MCAO조화SF조채용경내동맥니룡선선전법치우측대뇌중동맥전새120 min.관찰재관주후24 h시뇌조직병이학개변,검측열휴극단백(HSP)70화HSP90표체.결과 재관주24 h후,SF조뇌조직병이학손해경우MCAO조.Sham조미견HSP70급HSP90표체.MCAO조가견도교다적HSP70면역양성세포.명현고우Sham조(P<0.01);SF조HSP70면역양성세포분포교MCAO조엄범,수량명현증다,포장급포핵도가견HSP70면역양성세포[(1.341±0.464)vs.(0.856±0.574)](P<0.05),이SF조HSP90적표체여MCAO조상방(0.006±0.013)vs.(0.005±0.007)].결론 SF예처리가통과증가HSP70표체이대대서뇌결혈-재관주손상기보호작용.
Objective To investigate the effects of shenfu injection (SF) pretreatrnent on the expressions of heat shock protein(HSP)70 and HSP90 after cerebral ischemia-reperfusion(I-R) injury in rats. Methods Thirty male SD rats were randomized into three groups of ischemia by the middle cerebral artery occlusion for 120 min (MCAO), SF 10 ml/kg pretreatment (SF, 30 min before ischemia) and sham-operation(sham) with 10 rats each. The pathological changes and the expressions of HSP70 and HsP90 were detected at 24 h after reperfusion. Results The pathological changes was lower in group SF than that in group MCAO. The expressions of HSP70 and HSP90 were negative in group sham. The expression of HSP70 was higher in group SF than that in group MCAO at 24 h after reperfusionE(1. 341±0. 464) vs. (0. 856±0. 574)](P<0. 05). There was no significant difference in the levels of HSP90 expression between groups of SF and MCAOF(0. 006 ± 0. 013) vs. (0. 005 ±0. 007)]. Conclusion SF pretreatment increases the expression of HSP70, which benefits cerebral protection after I-R injury in rats.