CAJ | 학술논문

对人锰超氧化物歧化酶(human manganese superoxide dismutase,hMn-SOD)基因剪接异构体进行分析,并检测异构体的表达情况.在GenBank库中检索人锰超氧化物歧化酶基因异构体及编码基因组序列,利用Vector NTI 9生物软件进行核酸及蛋白序列比对;利用RT-PCR方法分析锰超氧化物歧化酶基因异构体的表达.结果显示,在GenBank库检索发现有3种人锰超氧化物歧化酶基因异构体,剪接异构的类型为可变的5′剪接位点和外显子盒,各异构体基因内含子均符合"GT-AG"规则.3种基因异构体编码两种异构体蛋白,即222个氨基酸的人锰超氧化物歧化酶蛋白以及中部缺少39个氨基酸的截短型异构蛋白.RT-PCR检测结果表明,剪接异构体hMn-SODb在HEK293T和HSC细胞中的表达比在HepG 2细胞中高,未见异构体hMn-SODc的表达.
대인맹초양화물기화매(human manganese superoxide dismutase,hMn-SOD)기인전접이구체진행분석,병검측이구체적표체정황.재GenBank고중검색인맹초양화물기화매기인이구체급편마기인조서렬,이용Vector NTI 9생물연건진행핵산급단백서렬비대;이용RT-PCR방법분석맹초양화물기화매기인이구체적표체.결과현시,재GenBank고검색발현유3충인맹초양화물기화매기인이구체,전접이구적류형위가변적5′전접위점화외현자합,각이구체기인내함자균부합"GT-AG"규칙.3충기인이구체편마량충이구체단백,즉222개안기산적인맹초양화물기화매단백이급중부결소39개안기산적절단형이구단백.RT-PCR검측결과표명,전접이구체hMn-SODb재HEK293T화HSC세포중적표체비재HepG 2세포중고,미견이구체hMn-SODc적표체.
It was to analyze the sequence of human manganese superoxide dismutase gene(hMn-SOD)splicing isoforms,and to detect the expression of hMn-SOD splicing isoforms The sequence of hMn-SOD transcript splicing isoforms and its genomic sequence were obtained by searching GenBank,and alignment of hMn-SOD transcript isoforms nucleotide sequences and coded amino acid sequences were carried out by Vector NTI 9 software RT-PCR was employed to analyze expression of the hMn-SOD transcript isoforms Results indicate that there are three hMn-SOD splicing isoforms in Genbank The types of alternative splicing are alternative 5′ splicing sites and cassette exons All sequences at the exon-intron junctions in all isoforms of hMn-SOD mRNA are consistent with the GT-AG rule Three isoforms of hMn-SOD mRNA encode two hMn-SOD isoforms,a 222 amino acid protein and a 39-amino acid truncated protein RT-PCR results showed that the level of hMn-SODb in HEK293T and HSC was higher than in HepG 2 cell and hMn-SODc isoform was not detected in HEK293T,HepG 2 and HSC cell There are three hMn-SOD splicing isoforms in GenBank The types of splicing isoforms are alternative 5′ splicing sites and cassette exons hMn-SODb mRNA was detected in HEK293T,HepG 2 and HSC cell,but hMn-SODc isoform was not detected.