CAJ | 학술논문

目的研究小窝蛋白-1(caveolin-1)在人胶质瘤中的表达,探讨其在胶质瘤的发生、发展及细胞增殖中的作用.方法应用免疫组化SP法检测80例胶质瘤和9例正常脑组织中caveolin-1与增殖细胞核抗原(PCNA)的表达情况.结果 caveolin-1在正常脑组织中全部阳性表达.80例胶质瘤中39例caveolin-1呈阳性表达,其在低级别胶质瘤中的阳性表达率高于高级别胶质瘤(P＜0.05);PCNA在正常脑组织中未见表达,80例胶质瘤中55例呈阳性表达,其在高级别胶质瘤中的阳性表达率高于低级别胶质瘤(P＜0.05).caveolin-1与PCNA在胶质瘤中的表达呈负相关性(r=-0.368,P＜0.01).结论 caveolin-1在胶质瘤中的表达对肿瘤细胞增殖起抑制作用,为胶质瘤的基因治疗提供了一个新的靶点.PCNA可作为反映胶质瘤细胞增殖、恶性程度及预后的指标之一.
목적 연구소와단백-1(caveolin-1)재인효질류중적표체,탐토기재효질류적발생、발전급세포증식중적작용.방법 응용면역조화SP법검측80례효질류화9례정상뇌조직중caveolin-1여증식세포핵항원(PCNA)적표체정황.결과 caveolin-1재정상뇌조직중전부양성표체.80례효질류중39례caveolin-1정양성표체,기재저급별효질류중적양성표체솔고우고급별효질류(P＜0.05);PCNA재정상뇌조직중미견표체,80례효질류중55례정양성표체,기재고급별효질류중적양성표체솔고우저급별효질류(P＜0.05).caveolin-1여PCNA재효질류중적표체정부상관성(r=-0.368,P＜0.01).결론 caveolin-1재효질류중적표체대종류세포증식기억제작용,위효질류적기인치료제공료일개신적파점.PCNA가작위반영효질류세포증식、악성정도급예후적지표지일.
Objective To explore the expression of caveolin-1 in human gliomas and its effects on the development and cell proliferation of gliomas.Methods The expression and distribution of caveolin-1 and proliferating cell nuclear antigen (PCNA) were determined by immunohistochemical technique in 80 cases of glioma specimens and 9 normal brain tissues.Results Caveolin-1 was expressed positively in all normal brain tissues,and in 39 of 80 cases of glioma specimens,higher in low grade glioma specimens than in high (P＜0.05).PCNA was not expressed in normal brain tissues,but expressed in 55 cases of glioma specimens,higher in high grade glioma than in low (P＜0.05).There was negative correlation between expressions of caveolin-1 and PCNA in glioma(r=-0.368,P＜0.01).Conclusion Caveolin-1 in glioma has depressant effects on tumor cell proliferation,providing a new target for the glioma gene therapy.PCNA can be used as one of prognostic indicators reflecting glioma cell proliferation,malignant degree and its prognosis.