中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2009年
3期
269-271
,共3页
牛会琴%陈昌辉%裴雪梅%吴青%李茂军
牛會琴%陳昌輝%裴雪梅%吳青%李茂軍
우회금%진창휘%배설매%오청%리무군
胆红素%新生儿%脐血%单核细胞%凋亡
膽紅素%新生兒%臍血%單覈細胞%凋亡
담홍소%신생인%제혈%단핵세포%조망
Bilirubin%Neonate%Cord blood%Monocyte%Apoptosis
目的 探讨胆红素对新生儿脐血单核细胞(CBMC)凋亡的影响.方法 采用纤维连接蛋白结合法分离新生儿CBMC.经含有不同浓度胆红素(6 mG/dl、9 mg/dl、12.9 mg/dl和18 mg/dl)的牛血清白蛋白溶液孵育1 h,再给予脂多糖(LPS,1μg/m1)刺激48 h,收集细胞.采用TUNEL法检测细胞的凋亡率.结果 LPS和低浓度(6 mg/dl)胆红素单独作用对CBMC的凋亡率均无影响.低浓度胆红素(6 mg/dl)与LPS共同作用不升高CBMC的凋亡率;较高浓度和高浓度胆红素(9 mg/dl、12.9 mg/dl、18 mg/dl)与LPS共同作用可升高CBMC的凋亡率,且这种作用随胆红索浓度的升高而增加.结论 较高浓度和高浓度胆红素可以升高CBMC的凋亡率,此种抑制作用可能与胆红素引起细胞膜破坏、Ca2+内流增多、线粒体功能障碍有关.
目的 探討膽紅素對新生兒臍血單覈細胞(CBMC)凋亡的影響.方法 採用纖維連接蛋白結閤法分離新生兒CBMC.經含有不同濃度膽紅素(6 mG/dl、9 mg/dl、12.9 mg/dl和18 mg/dl)的牛血清白蛋白溶液孵育1 h,再給予脂多糖(LPS,1μg/m1)刺激48 h,收集細胞.採用TUNEL法檢測細胞的凋亡率.結果 LPS和低濃度(6 mg/dl)膽紅素單獨作用對CBMC的凋亡率均無影響.低濃度膽紅素(6 mg/dl)與LPS共同作用不升高CBMC的凋亡率;較高濃度和高濃度膽紅素(9 mg/dl、12.9 mg/dl、18 mg/dl)與LPS共同作用可升高CBMC的凋亡率,且這種作用隨膽紅索濃度的升高而增加.結論 較高濃度和高濃度膽紅素可以升高CBMC的凋亡率,此種抑製作用可能與膽紅素引起細胞膜破壞、Ca2+內流增多、線粒體功能障礙有關.
목적 탐토담홍소대신생인제혈단핵세포(CBMC)조망적영향.방법 채용섬유련접단백결합법분리신생인CBMC.경함유불동농도담홍소(6 mG/dl、9 mg/dl、12.9 mg/dl화18 mg/dl)적우혈청백단백용액부육1 h,재급여지다당(LPS,1μg/m1)자격48 h,수집세포.채용TUNEL법검측세포적조망솔.결과 LPS화저농도(6 mg/dl)담홍소단독작용대CBMC적조망솔균무영향.저농도담홍소(6 mg/dl)여LPS공동작용불승고CBMC적조망솔;교고농도화고농도담홍소(9 mg/dl、12.9 mg/dl、18 mg/dl)여LPS공동작용가승고CBMC적조망솔,차저충작용수담홍색농도적승고이증가.결론 교고농도화고농도담홍소가이승고CBMC적조망솔,차충억제작용가능여담홍소인기세포막파배、Ca2+내류증다、선립체공능장애유관.
Objective To explore the effects of bilirubin on the apoptosis index(AI) of cord blood monocyte(CBMC).Methods The CBMC of normal full-term neonates selected randomly as subjects was isolated by the method of gelatin/plasma coated flasks.The CBMC was preincubated with unconjugated bilirubin dissolved in bovine albumin solution at various concentration(6 mg/dl、9 mg/dl、 12.9 mg/dl and 18 mg/dl).Bilirubin-treated CBMC was further cultured with LPS for inducing cellular activation for 48 hours,and then the CBMC was collected.The AI of CBMC after cultivation was detected by using TUNEL methods.Results No differences of AI among the normal control group,LPS group and 6 mg/dl (free-LPS) group(P ＞ 0.05) were found.In the presence of LPS,the AI of the 6 mg/dl group was similar to those of the normal control group,LPS group and 6 mg/dl(free-LPS) group(P ＞0.05),while the AIs of the 9 mg/dl group,12 mg/dl group and 18 mg/dl group were significantly higher than those of the normal control group,LPS group,6 mg/dl(free-LPS) group and 6 mg/dl group(P＜0.05).The increase of the AI of CBMC was corresponding to the raise of the bilirubin concentration.Conclusion The bilirubin can increase the AI of CBMC by destroing the configuration of the cell membrane,increasing intracellular calcium concentration and inducing the mitochondrial energy failure.
