CAJ | 학술논문

目的研究高迁移率族蛋白1( high mobility group box-1,HMGB1) mRNA及蛋白在喉鳞状细胞癌(简称喉鳞癌)组织及患者血清中的表达及其临床意义.方法反转录聚合酶链反应( RTPCR)及Western blot分别检测HMGB1 mRNA及蛋白在30例喉鳞癌患者肿瘤组织及癌旁黏膜组织中的表达；酶联免疫吸附试验( ELISA)检测HMGB1蛋白在喉鳞癌患者及正常志愿者血清中的表达.结果RT-PCR结果示HMGB1 mRNA(HMGB1/GAPDH)在喉鳞癌组织、癌旁黏膜组织中的相对表达量((x)±s)分别为1.25±0.12、0.32±0.04,差异有统计学意义(t=40.27,P＜0.05)；Western blot结果示HMGB1蛋白(HMGB1/β-actin)在喉鳞癌和癌旁黏膜组织中的相对表达量分别为1.29 ±0.10、0.34±0.03,两组间差异有统计学意义(t=49.84,P＜0.05).结果显示HMGB1 mRNA和蛋白的表达水平与患者的T分级、临床分期、有无淋巴转移及是否吸烟有关(P值均＜0.05)；而与年龄、性别、是否饮酒及肿瘤病理分级无关(P＞0.05).ELISA结果显示,喉鳞癌患者及正常志愿者血清中HMGB1质量浓度分别为(24.80±14.08) ng/ml、(23.58±14.69)ng/ml,差异无统计学意义(t=0.37,P＞0.05).结论 HMGB1 mRNA及蛋白在喉鳞癌组织中的表达显著高于癌旁黏膜组织,且表达水平与T分级、临床分期及有无淋巴转移有关.
목적 연구고천이솔족단백1( high mobility group box-1,HMGB1) mRNA급단백재후린상세포암(간칭후린암)조직급환자혈청중적표체급기림상의의.방법 반전록취합매련반응( RTPCR)급Western blot분별검측HMGB1 mRNA급단백재30례후린암환자종류조직급암방점막조직중적표체；매련면역흡부시험( ELISA)검측HMGB1단백재후린암환자급정상지원자혈청중적표체.결과RT-PCR결과시HMGB1 mRNA(HMGB1/GAPDH)재후린암조직、암방점막조직중적상대표체량((x)±s)분별위1.25±0.12、0.32±0.04,차이유통계학의의(t=40.27,P＜0.05)；Western blot결과시HMGB1단백(HMGB1/β-actin)재후린암화암방점막조직중적상대표체량분별위1.29 ±0.10、0.34±0.03,량조간차이유통계학의의(t=49.84,P＜0.05).결과현시HMGB1 mRNA화단백적표체수평여환자적T분급、림상분기、유무림파전이급시부흡연유관(P치균＜0.05)；이여년령、성별、시부음주급종류병리분급무관(P＞0.05).ELISA결과현시,후린암환자급정상지원자혈청중HMGB1질량농도분별위(24.80±14.08) ng/ml、(23.58±14.69)ng/ml,차이무통계학의의(t=0.37,P＞0.05).결론 HMGB1 mRNA급단백재후린암조직중적표체현저고우암방점막조직,차표체수평여T분급、림상분기급유무림파전이유관.
Objective To investigate the mRNA and protein expressions of high mobility group box-1 ( HMGB1 ) in the tumor tissues and sera of patients with laryngeal squamous cell carcinoma (LSCC) and their clinical significance. Methods Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were used to detected the expressions of HMGB1 mRNA and protein in the tumors and adjacent normal epithelial tissues in 30 patients with LSCC.Serum HMGB1 protein levels in the patients with LSCC and in 10 healthy volunteers were detected with enzyme-linked immunosorbent adsorption experiment( ELISA ).Results RT-PCR demonstrated that the mean relative mRNA expression levels of HMGB1 (HMGB1/GAPDH) in LSCC tissues and in adjacent normal epithelial tissues were 1.25 ±0.12 and 0.32 ± 0.04,respectively ( t =40.27,P ＜ 0.05 ).Western blot revealed that the mean relative protein expression levels of H MGB 1 ( HMGB1/β-actin) were 1.29 ± 0.10 and 0.34 ± 0.03 (t =49.84,P ＜ 0.05 ),respectively.Both mRNA and protein expression levels of HMGB1 were associated with T stage,clinical stage,lymph node metastasis status and smoking ( all P ＜ 0.05 ),but no significant correlation with age,alcohol consumption and primary tumor grade and location (all P ＞0.05 ).Mean serum HMGB1 protein levels in patients with LSCC and healthy volunteers were (24.80 ± 14.08) ng/ml and (23.58 ± 14.69) ng/ml ( t =0.37,P ＞ 0.05 ).Conclusions Both mRNA and protein expressions of HMGB1 were obviously elevated in LSCC,which were associated closely with T stage,clinical stage and lymph node metastasis.