CAJ | 학술논문

目的研究重型颅脑交通伤患者脑挫伤组织核心区、边缘区水孔蛋白质-4(AQP-4)的表达水平及其意义.方法选取2007年1月-2009年7月我科重型颅脑交通伤手术患者(额颞区脑挫伤)30例,按受伤至手术时间长短平均分为O～4 h组(A组)、4～8 h组(B组)和8～12 h组(C组).每位患者术中分别取挫伤组织边缘区及核心区组织各一.以桥小脑区肿瘤切除术中为获取手术通道而切除的5例患者正常脑组织并分成10份作为对照,应用Western-blot方法,以GAPDH为内参,分别检测边缘区、核心区挫伤组织及正常脑组织中AQP-4的表达水平.结果边缘区组织AQP-4表达随时间增加而逐步升高,也明显高于核心区和正常组的表达水平,而核心区组织中AQP-4的表达低于正常脑组织.结论边缘区AQP-4的高表达在伤后早期即开始,且有明显的时间依赖性.而核心区AQP-4的表达水平较低可能与血脑屏障及其周围结构的早期破坏有关.
목적 연구중형로뇌교통상환자뇌좌상조직핵심구、변연구수공단백질-4(AQP-4)적표체수평급기의의.방법 선취2007년1월-2009년7월아과중형로뇌교통상수술환자(액섭구뇌좌상)30례,안수상지수술시간장단평균분위O～4 h조(A조)、4～8 h조(B조)화8～12 h조(C조).매위환자술중분별취좌상조직변연구급핵심구조직각일.이교소뇌구종류절제술중위획취수술통도이절제적5례환자정상뇌조직병분성10빈작위대조,응용Western-blot방법,이GAPDH위내삼,분별검측변연구、핵심구좌상조직급정상뇌조직중AQP-4적표체수평.결과 변연구조직AQP-4표체수시간증가이축보승고,야명현고우핵심구화정상조적표체수평,이핵심구조직중AQP-4적표체저우정상뇌조직.결론 변연구AQP-4적고표체재상후조기즉개시,차유명현적시간의뢰성.이핵심구AQP-4적표체수평교저가능여혈뇌병장급기주위결구적조기파배유관.
Objective To study the expression of aquaporin-4 (AQP-4) in core and marginal region of the contusion brain tissues from patients with severe traffic brain injuries. Methods Thirty patients with severe traffic brain injuries (frontal-temporal brain contusion) admitted into our department from January 2007 to July 2009 were enrolled in the study and divided into three groups according to the period from injury to operation, ie, 0-4 hours (Group A), 5-8 hours (Group B) and 9-12 hours (Group C). The tissue was collected from core and marginal regions of brain contusion in each group. Ten parts of normal brain tissues obtained from the cerebellum to gain good exposure of CPA region tumors were used as control. The expression of AQP-4 in the normal brain tissues and in the tissues from core and marginal region of brain contusion, with GAPDH used as a control. Results The expression of AQP-4 in the marginal region was distinctly higher than that in normal tissues and in the tissues from core region. The AQP-4 expression in the tissues of the core region was lower than that in normal tissues. Conclusions AQP-4 is highly expressed in the tissues from the marginal region early after injury in a time-dependent fashion. Low expression level of AQP-4 in the core region is possibly correlated with early damage of blood brain barrier and peripheral structures.